Human proinsulin (PI) ELISA kit (12.5-800 pg/mL)

Cat. No.: 0126WXX-1274

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PI

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Human proinsulin (PI) ELISA kit (12.5-800 pg/mL)

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Product Overview

Description: Human proinsulin (PI) ELISA kit (12.5-800 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PI in human samples with a range of 12.5-800 pg/mL and a minimum detectable dose (sensitivity) of <4.7 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The provided microtiter plate is pre-coated with an antibody specific to PI. Standards or samples are introduced into the wells along with a biotin-conjugated detection antibody specific to PI. Subsequently, avidin-conjugated HRP is added to each well and incubated. After the addition of TMB substrate, color changes occur only in wells containing the PI-biotin-avidin complex. The reaction is stopped with a sulphuric acid solution, and the absorbance is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of PI is determined by comparing the sample O.D. to the standard curve.

Background: Proinsulin is the precursor molecule of insulin, synthesized in pancreatic β cells and subsequently processed into mature insulin and C-peptide. It plays a critical role in glucose homeostasis by serving as an essential intermediate in insulin biosynthesis and secretion. Under normal physiological conditions, proinsulin is efficiently converted to insulin; however, impaired processing or increased proinsulin secretion reflects β-cell dysfunction. Elevated circulating proinsulin levels are commonly observed in obesity and insulin resistance, making proinsulin a key indicator of metabolic stress and a contributing factor to the development of obesity-associated type 2 diabetes.

Synonyms: INS; Pro-Insulin; Preproinsulin

Applications: Human proinsulin (PI) ELISA kit (12.5-800 pg/mL) is used to quantify PI in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids of humans, providing data to support research in a wide range of areas, including endocrinology, hormone metabolism, obesity, and others.

Research Area: Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 12.5-800 pg/mL

Sensitivity: <4.7 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (standard; detection reagent A; detection reagent B; 96-well strip plate) and 4°C (others).

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