Human lipoprotein lipase (LPL) ELISA kit (3.12-200 ng/mL)

Cat. No.: 0126WXX-947

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LPL

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Human lipoprotein lipase (LPL) ELISA kit (3.12-200 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human lipoprotein lipase (LPL) ELISA kit (3.12-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in human samples with a range of 3.12-200 ng/mL and a minimum detectable dose (sensitivity) of <0.6 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the double-antibody sandwich ELISA technique. The microplate is pre-coated with an anti-human LPL monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added and subsequently washed with PBS or TBS. Avidin-peroxidase conjugates are then added to the wells. TMB substrate is used for coloration after the unbound conjugates are washed out. TMB forms a blue product that turns yellow after adding the stop solution (color reagent C). The color intensity is positively correlated with the quantity of target analyte in the sample.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Human lipoprotein lipase (LPL) ELISA kit (3.12-200 ng/mL) is used to quantify LPL in serum, plasma, cell culture supernatant of human, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity