Human insulin receptor substrate 1 (IRS-1) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-700

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: IRS-1

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Human insulin receptor substrate 1 (IRS-1) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human insulin receptor substrate 1 (IRS-1) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IRS-1 in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.094 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes the Sandwich-ELISA methodology for the quantification of IRS1. The provided micro ELISA plate is pre-coated with a capture antibody specific to IRS1. Standards or samples are added to the wells to bind with the immobilized antibody. A biotinylated detection antibody specific for IRS1 and an avidin-HRP conjugate are then added sequentially and incubated. After washing away unbound components, a substrate solution is introduced. Wells containing the IRS1 complex appear blue and shift to yellow after the reaction is halted with a sulphuric acid solution. The optical density (O.D.), recorded at 450 nm ± 2 nm, is directly proportional to the IRS1 concentration.

Background: IRS-1 is a key adaptor protein that plays a central role in insulin signaling. When insulin binds to its receptor on the cell surface, the receptor is activated and phosphorylates tyrosine residues on IRS-1. This process transforms IRS-1 into a signaling “relay station,” enabling it to bind with multiple downstream signaling proteins such as phosphoinositide 3-kinase (PI3K). IRS-1 transmits insulin signals from the cell membrane into the cell interior, thereby initiating a series of vital biological responses, including promoting glucose transport and glycogen synthesis. Studying the function and defects of IRS-1 is crucial for understanding the pathological processes underlying obesity.

Synonyms: IRS1

Applications: Human insulin receptor substrate 1 (IRS-1) ELISA kit (0.156-10 ng/mL) is used to quantify IRS-1 in serum, plasma, and biological fluids of human, providing data to support research in a wide range of areas, including endocrinology, obesity, etc.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.094 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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