Human hormone-sensitive lipase (HSL) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-888

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: HSL

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Human hormone-sensitive lipase (HSL) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human hormone-sensitive lipase (HSL) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSL in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.065 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The methodology applied in this kit is the sandwich enzyme immunoassay. The microtiter plate is pre-immobilized with an antibody specific to HSL. Standards or samples are added to the appropriate wells with a biotin-conjugated detection antibody specific for HSL. Next, avidin-conjugated HRP is added to each microplate well and incubated. After TMB substrate addition, color development occurs exclusively in wells containing the HSL sandwich complex. The reaction is halted by a sulphuric acid solution, and the color change is recorded at 450 nm ± 10 nm. The HSL concentration is calculated by mapping the O.D. of the samples against the established standard curve.

Background: HSL is the key enzyme for fat breakdown. Belonging to the “GDXG” lipase family, this enzyme primarily hydrolyzes stored triacylglycerols into free fatty acids in adipose tissue and the heart. This process represents the rate-limiting step of lipolysis, controlling the overall speed of fat breakdown. HSL can be rapidly activated by catecholamine hormones (such as epinephrine) through a cyclic adenosine monophosphate (cAMP)-dependent phosphorylation process, while insulin regulates its dephosphorylation and inactivation. Due to its functional characteristics, HSL has become a central subject in obesity research, particularly in the regulation of fat storage and release.

Synonyms: LHS; LIPE; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Human hormone-sensitive lipase (HSL) ELISA kit (0.156-10 ng/mL) is used to quantify HSL in serum, plasma, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolic pathway, obesity, etc.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.065 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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