Human glucagon (GCG) ELISA kit (12.9-200 pg/mL)

Cat. No.: 0126WXX-89

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: GCG

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Human glucagon (GCG) ELISA kit (12.9-200 pg/mL)

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Product Overview

Description: Human glucagon (GCG) ELISA kit (12.9-200 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in human samples with a range of 12.9-200 pg/mL and a minimum detectable dose (sensitivity) of 4.3 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit uses a microplate pre-coated with a capture antibody specific to GCG. Standards or samples are added along with an HRP-conjugated monoclonal detection antibody, allowing GCG to be simultaneously captured and bound by the HRP-conjugate, forming a sandwich. After incubation, the wells are washed. A substrate solution is then added, and the resulting color is measured at 450 nm after the reaction is stopped. The intensity of the color is directly proportional to the amount of GCG captured, and concentrations are determined via comparison to a standard curve.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Human glucagon (GCG) ELISA kit (12.9-200 pg/mL) is used to quantify GCG in serum, plasma, and tissue homogenates of human, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 12.9-200 pg/mL

Sensitivity: 4.3 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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