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Human cathepsin S (CTSS) ELISA kit (0.5-10 ng/mL)
Cat. No.:
OB0625WXX-275
Assay Type:
Quantitative competitive or sandwich ELISA
Target Species:
Human
Assay Target:
CTSS
Size:
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Product Overview
Description:
Human cathepsin S (CTSS) ELISA kit (0.5-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSS in human with a range of 0.5-10 ng/mL and a specificity of 0.1 ng/mL.
Assay Principle:
The microtiter plate is pre-coated with a monoclonal antibody specific for CTSS. Standards or samples are then added to the wells of the microtiter plate, and CTSS (if present) will bind to the wells pre-coated with the antibody. To quantify the amount of CTSS in the sample, a standard preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for CTSS is added to each well to “clamp” CTSS immobilized on the microtiter plate. The microtiter plate is incubated, and the wells are then washed thoroughly to remove all unbound components. The substrate solution is then added to each well. The enzyme (HRP) and substrate will react during the short incubation period. Only wells containing CTSS and enzyme-bound antibody will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm. A standard curve of color intensity (O.D.) versus concentration of the standard is plotted. The concentration of CTSS in each sample is derived from this standard curve.
Background:
CTSS is an important member of the cysteine protease family. As a potent mammalian elastase, it can degrade various extracellular components, including fibronectin, laminin, elastin, and collagen. CTSS is abnormally expressed in various disease states, such as obesity and atherosclerosis, which makes it a biomarker and a potential therapeutic target. Studies have found that CTSS inhibitors may be a possible strategy for treating obesity.
Synonyms:
CTS-S; EC 3.4.22.27
EC NO.:
3.4.22.27
Applications:
Human cathepsin S (CTSS) ELISA kit (0.5-10 ng/mL) is used to quantify CTSS in serum, plasma, cell culture supernatants, body fluid, tissue homogenate samples of human, providing data to support a wide range of studies.
Research Area:
Obesity research; Immune response; Signaling pathway; Protein degradation
Specification
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range:
0.5-10 ng/mL
Sensitivity:
0.1 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.