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Vitamin B12 test kit

Human 2, 4-dienoyl-CoA reductase (DECR1) ELISA kit

Cat. No.: OB0625WXX-578
Assay Type: Quantitative sandwich ELISA
Target Species: Human
Assay Target: DECR1
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Human 2, 4-dienoyl-CoA reductase (DECR1) ELISA kit
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Product Overview

Description: Human 2, 4-dienoyl-CoA reductase (DECR1) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of DECR1 in human with a range of 0.312-20 ng/mL and a specificity of 0.06 ng/mL.
Assay Principle: The pre-coated antibody is Human DECR1 monoclonal antibody, and the detection antibody is biotin-labeled polyclonal antibody. The samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells. The reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue under the catalysis of peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
Background: DECR1 is a mitochondrial and peroxisomal enzyme essential for the complete beta-oxidation of unsaturated fatty acids.
Synonyms: EC 1.3.1.34; 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial; 2,4-dienoyl-CoA reductase 1
EC NO.: 1.3.1.34
Applications: Human 2, 4-dienoyl-CoA reductase (DECR1) ELISA kit is used to quantify DECR1 in human serum, plasma, cell culture supernatant, and tissue samples of human, providing data to support a wide range of studies.
Research Area: Lipid metabolism; Obesity research

Specification

Sample Type: Human serum; Plasma; Cell culture supernatant; Organizations
Detection Range: 0.312-20 ng/mL
Sensitivity: 0.06 ng/mL
Precision (Intra-assay): CV≤8%
Precision (Inter-assay): CV≤12%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.
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