Guinea pig glucocorticoid receptor-beta (GR-β) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-294

Assay Type: Quantitative competitive ELISA

Target Species: Guinea pig

Assay Target: GR-β

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Guinea pig glucocorticoid receptor-beta (GR-β) ELISA kit-Quantitative competitive

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Product Overview

Description: Guinea pig glucocorticoid receptor-beta (GR-β) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GR-β in guinea pig samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The GR-β ELISA kit utilizes a competitive enzyme immunoassay technique featuring a monoclonal anti-GR-β antibody and a GR-β-HRP conjugate. Samples and assay buffer are incubated with the GR-β-HRP conjugate in a pre-coated plate for one hour. During this period, endogenous GR-β from the sample competes with the HRP-conjugate for a limited number of antibody binding sites. After incubation, the wells are decanted and washed five times. An HRP substrate is then added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm, where the color intensity is inversely proportional to the GR-β concentration. A standard curve is generated to relate the O.D. to standard concentrations for sample interpolation.

Background: GR-β is a splice isoform of the glucocorticoid receptor (GR). Unlike the physiologically active GR-α we are familiar with, GR-β cannot bind glucocorticoids and thus cannot mediate the classical glucocorticoid signaling pathway. Its primary function is believed to be the negative regulation of GR-α activity. It can form heterodimers with GR-α, preventing GR-α from entering the cell nucleus and binding to DNA, thereby inhibiting GR-α-mediated gene transcription. Consequently, GR-β is regarded as an endogenous inhibitor or antagonist of glucocorticoid action. Changes in GR-β levels may be associated with diseases such as obesity.

Synonyms: GRb; GR-beta

Applications: Guinea pig glucocorticoid receptor-beta (GR-β) ELISA kit is used to quantify GR-β in serum, plasma, cell culture superna tants, body fluid, and tissue homogenate of guinea pig, providing data to support research in a wide range of areas, including signal transduction, endocrinology, hormone metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture superna tants; Body fluid; Tissue homogenate

Detection Range: 250-5000 pg/mL

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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