Chicken ileal fatty acid binding protein 6 (FABP6) ELISA kit

Cat. No.: OB0625WXX-814

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: FABP6

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Chicken ileal fatty acid binding protein 6 (FABP6) ELISA kit

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Product Overview

Description: Chicken ileal fatty acid binding protein 6 (FABP6) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FABP6 in chicken with a specificity of 0.1 ng/mL.

Assay Principle: The assay samples and buffer are incubated with FABP6-HRP conjugate in a pre-coated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with HRP enzyme substrate. The product of the enzyme reaction with the substrate forms a blue colored complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the FABP6 concentration because FABP6 in the sample competes with the FABP6-HRP conjugate for the binding site of the anti-FABP6 antibody. Since there are a limited number of binding sites, as more sites are occupied by FABP6 in the sample, there are fewer sites left to bind the FABP6-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The FABP6 concentration in each sample is derived from this standard curve.

Background: Ileal FABP6 is a protein highly expressed in the ileum that specifically binds and transports bile acids, playing a key role in the enterohepatic circulation.

Synonyms: Fatty acid binding protein 6, Ileal; GT; iBABP; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ILLBP; I-15P; Gastrotropin; Illeal lipid-binding protein; Ileal bile acid binding protein; Intestinal 15 kDa protein

Applications: Chicken ileal fatty acid binding protein 6 (FABP6) ELISA kit is an important tool for the quantitative detection of FABP6 in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of chicken.

Research Area: Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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