Chicken ghrelin (GHRL) ELISA kit (123.5-10000 pg/mL)

Cat. No.: 0126WXX-159

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: GHRL

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Chicken ghrelin (GHRL) ELISA kit (123.5-10000 pg/mL)

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Product Overview

Description: Chicken ghrelin (GHRL) ELISA kit (123.5-10000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GHRL in chicken samples with a range of 123.5-10000 pg/mL and a minimum detectable dose (sensitivity) of 48.4 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for GHRL is pre-coated onto a microplate. Endogenous GHRL from the sample and a fixed amount of biotin-labeled GHRL compete for binding to the immobilized antibody. After incubation and washing, an avidin-HRP conjugate is added to bind the captured biotinylated GHRL. Upon substrate addition, the resulting color intensity is inversely proportional to the concentration of GHRL in the sample. A higher concentration of sample GHRL results in less biotin-labeled GHRL binding, thereby producing a weaker color signal.

Background: Ghrelin is a peptide hormone primarily secreted by the stomach, exerting its effects by binding to the growth hormone secretagogue receptor type 1 (GHSR). It plays multiple roles in the body, but its most well-known function is regulating energy balance and appetite. Research indicates that ghrelin levels and their regulation of appetite may be abnormal in obese individuals. It is considered a key target for developing anti-obesity drugs.

Synonyms: MTLRP; Preproghrelin; Growth hormone secretagogue receptor ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Applications: Chicken ghrelin (GHRL) ELISA kit (123.5-10000 pg/mL) is used to quantify GHRL in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of chicken, providing data to support research in a wide range of areas, including signal transduction, endocrinology, gastroenterology, metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Gastroenterology; Metabolism; Obesity