Canine peptide YY (PYY) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1637

Assay Type: Quantitative competitive ELISA

Target Species: Canine

Assay Target: PYY

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Canine peptide YY (PYY) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine peptide YY (PYY) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in canine samples with a range of 50-1000 pg/mL and a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PYY ELISA Kit utilizes a competitive enzyme immunoassay technique. Samples and buffers are incubated with a PYY-HRP conjugate in a pre-coated plate for one hour, allowing endogenous PYY and the conjugate to compete for a limited number of binding sites on the polyclonal anti-PYY antibody. Following incubation, the wells are washed five times to remove unbound reagents. An HRP-specific substrate is then added to form a blue product that turns yellow upon the addition of a stop solution. The resulting optical density (O.D.), measured at 450 nm, is inversely proportional to the PYY concentration in the sample. A standard curve is plotted to interpolate the precise quantitative values for each sample.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Canine peptide YY (PYY) ELISA kit-Quantitative competitive is used to quantify PYY in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of canine, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 50-1000 pg/mL

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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