Canine ghrelin (GHRL) ELISA kit (0.05-20 mg/mL)

Cat. No.: 0126WXX-156

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: GHRL

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Canine ghrelin (GHRL) ELISA kit (0.05-20 mg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine ghrelin (GHRL) ELISA kit (0.05-20 mg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GHRL in canine samples with a range of 0.05-20 mg/mL and a minimum detectable dose (sensitivity) of 0.019 mg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a microplate pre-coated with a canine GHRL capture antibody. GHRL in the sample is captured by the immobilized antibody, followed by the addition of a biotinylated canine GHRL detection antibody to form a "sandwich" complex. Streptavidin-HRP is then added to bind the biotinylated antibody. After washing, a substrate solution is introduced; color develops in direct proportion to the amount of GHRL present. The reaction is terminated with an acidic stop solution and measured at 450 nm.

Background: Ghrelin is a peptide hormone primarily secreted by the stomach, exerting its effects by binding to the growth hormone secretagogue receptor type 1 (GHSR). It plays multiple roles in the body, but its most well-known function is regulating energy balance and appetite. Research indicates that ghrelin levels and their regulation of appetite may be abnormal in obese individuals. It is considered a key target for developing anti-obesity drugs.

Synonyms: MTLRP; Preproghrelin; Growth hormone secretagogue receptor ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Applications: Canine ghrelin (GHRL) ELISA kit (0.05-20 mg/mL) is used to quantify GHRL in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of canine, providing data to support research in a wide range of areas, including signal transduction, endocrinology, gastroenterology, metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Gastroenterology; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.05-20 mg/mL

Sensitivity: 0.019 mg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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