Bovine growth hormone (GH) ELISA kit (78.125-5000 pg/mL)

Cat. No.: 0126WXX-95

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: GH

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Bovine growth hormone (GH) ELISA kit (78.125-5000 pg/mL)

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Product Overview

Description: Bovine growth hormone (GH) ELISA kit (78.125-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in bovine samples with a range of 78.125-5000 pg/mL and a minimum detectable dose (sensitivity) of 28 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to GH. Standards or samples are added to the wells along with a biotin-conjugated detection antibody. Subsequently, Avidin-HRP is added and incubated. Upon addition of TMB substrate, only wells containing the GH sandwich complex will exhibit a color change. The reaction is terminated with sulphuric acid and measured spectrophotometrically at 450 nm ± 10 nm. The GH concentration is determined by comparing the O.D. of the samples to the standard curve.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Bovine growth hormone (GH) ELISA kit (78.125-5000 pg/mL) is used to quantify GH in serum, plasma, tissue homogenates, and other biological fluids of bovine, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Other biological fluids

Detection Range: 78.125-5000 pg/mL

Sensitivity: 28 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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