Bovine adiponectin (ADP) ELISA kit (78.125-5000 pg/mL)

Cat. No.: OB0525WXX-248

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: ADP

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Bovine adiponectin (ADP) ELISA kit (78.125-5000 pg/mL)

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Product Overview

Description: Bovine adiponectin (ADP) ELISA kit (78.125-5000 pg/mL) is a ready-to-use ELISA kit for analyzing bovine ADP levels with a detection range of 78.125-5000 pg/mL and a sensitivity of 32 pg/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with a specific ADP antibody. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADP. Avidin conjugated to HRP is then added to each microplate well and incubated. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Bovine adiponectin (ADP) ELISA kit (78.125-5000 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in bovine serum, plasma, and other biological fluid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 78.125-5000 pg/mL

Sensitivity: 32 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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