Overview of Gene Editing-based In Vivo Obesity Model Development

As obesity becomes a global health challenge, Protheragen is well aware of the importance of accurate research models in promoting the analysis of obesity mechanisms and the development of anti-obesity drugs. Therefore, we are proud to provide gene editing-based in vivo obesity model development service, which is based on our deep accumulation of gene editing technology and provides powerful research tools for the scientific research community through customized construction of in vivo obesity models. This service is not only a natural extension of our Preclinical Studies of Anti-Obesity Therapeutics but also a key link in our Obesity Models and In Vivo Obesity Models for Obesity Research service. By accurately simulating the pathophysiological process of obesity, our model provides a valuable platform for evaluating the efficacy and safety of anti-obesity drugs.

We specialize in providing gene editing-based in vivo obesity model development services to advance Anti-Obesity Therapy Development. Through precision gene editing techniques, we create obesity models that mimic specific genetic mutations linked to obesity in humans. These models allow us to assess the therapeutic potential of our candidate treatments, including Anti-Obesity Small Molecule Drug Development and emerging Anti-Obesity Gene Therapy Development.

Revolutionize Obesity Research with Our Cutting-Edge Gene Editing In Vivo Model

Gene editing technology has transformed biomedical research, allowing for precise alterations to the DNA of an organism. This precision is particularly beneficial in developing in vivo models of obesity, as it enables the targeting of specific genes involved in obesity regulation. We leverage advanced gene editing techniques to create customized obesity models that provide invaluable insights into the genetic bases of obesity and potential therapeutic interventions.

• Gene Editing Tools and Strategies

  Based on research needs, we carefully select advanced gene editing tools and design specific sgRNA structures to ensure accurate editing of target genes. The selection of these tools is based on the sequence characteristics of the target gene, editing efficiency, potential off-target effects, and comprehensive consideration of the research objectives.

• Diverse In Vivo Models

  Mouse Model: As the most commonly used model organism, mice have the advantages of rapid reproduction, clear genetic background, and easy operation, making them our first choice for building obesity models. Through gene editing technology, we simulate human obesity-related gene mutations in mice and conduct in-depth research on the pathogenesis of obesity and the effects of drug treatment.

  Rat Model: Compared with mice, rats are closer to humans in terms of body shape, metabolic rate, and drug response, so they are also an important choice for building obesity models. We use gene editing technology to create specific obesity phenotypes in rats to evaluate the effectiveness and safety of anti-obesity drugs.

  Dog Model: In certain specific studies, dogs have become valuable in vivo models because of their high similarity to humans in physiology, metabolism, and behavior. In our dog model, we simulate obesity-related lesions through gene editing technology, providing an important reference for the study of pet obesity and similar diseases in humans.

  Pig Model: Pigs have many similarities with humans in terms of body shape, anatomical structure, physiological functions, and metabolic processes, so they are regarded as "ideal models of human diseases". We use gene editing technology to construct obesity models in pigs to study the effects of obesity on the cardiovascular system, metabolic system, and other aspects.

  Rabbit Model: Although rabbits are relatively rarely used in obesity research, they have some unique advantages as experimental animals, such as sensitivity to certain drugs. Therefore, in certain cases, we also consider using rabbits as in vivo models for obesity research.

• Model Construction and Optimization

  We use advanced technologies such as microinjection and embryo transplantation to introduce gene editing tools into the fertilized eggs or embryonic stem cells of the above-mentioned model organisms to construct obesity model animals carrying specific gene editing. During the model construction process, we strictly control the experimental conditions to ensure the genetic stability and phenotypic consistency of the model animals. At the same time, we continuously optimize the model construction process to improve the efficiency and success rate of gene editing.

• Model Verification and Evaluation

  We conduct comprehensive phenotypic identification and physiological index monitoring of the constructed obesity model animals, including body weight, body fat percentage, food intake, energy consumption, metabolic rate, and blood biochemical indicators. In addition, we also use molecular biological methods to verify the gene editing effect of model animals to ensure the successful construction of the model and the realization of the expected phenotype.

• Customized Service

  We are well aware of the uniqueness and complexity of each research project, so we provide highly customized service solutions. From the selection of in vivo models and the design of gene editing strategies to the construction and verification of model animals, we always maintain close communication with clients to ensure that the service content fully meets client needs and expectations.

Workflow

We start by communicating with clients to clarify research goals and conduct feasibility assessments. Based on their needs, we design detailed gene editing and model construction plans, prepare high-quality gene editing tools, and establish gene-edited animal models through embryo microinjection and other techniques. We then verify the models, collect data, analyze the results, and compile detailed reports for clients. Our commitment extends beyond project completion, offering long-term feeding recommendations, technical support, and further collaboration opportunities.

Applications of Gene Editing-based In Vivo Obesity Model

• Drug development and screening: This service builds an in vivo model that accurately simulates the pathophysiological process of human obesity, providing an ideal testing platform for the development of new anti-obesity drugs.
• Obesity mechanism research: The obesity model constructed by gene editing technology helps to deeply study the molecular mechanism of obesity.
• Exploration of personalized medical solutions: This service can be used to build in vivo models carrying different obesity-related gene mutations to simulate the pathological conditions of different patients. This information helps researchers explore precise treatment plans for patients with different genotypes.

Advantages

• We use cutting-edge gene editing tools to ensure precise editing of target genes. This high precision not only improves the success rate of model construction but also ensures the stability and consistency of the phenotype of the model animals, laying a solid foundation for subsequent scientific research.
• We provide a variety of in vivo models including mice, rats, dogs, pigs, and rabbits to meet the needs of different scientific research projects. Each model has its own unique physiological and metabolic characteristics, which more comprehensively simulate the complex pathophysiological process of human obesity.
• We provide highly customized service solutions because we are fully aware of the uniqueness and complexity of each research project.

Publication

Our Services

Frequently Asked Questions

Why choose a gene-edited in vivo obesity model instead of a traditional model?

Gene-edited in vivo obesity models can more accurately simulate the obesity phenotype caused by specific gene mutations, which are often closely related to human obesity. Compared with traditional models, gene-edited models can reduce the interference of genetic background, making the research results more accurate and reliable. In addition, by editing specific genes, researchers can deeply explore the molecular mechanisms of obesity and provide strong support for the development of new therapies.

Does your company provide technical support If I encounter problems during the use of the model?

Yes, we provide clients with full technical support. You can contact our technical support team at any time if you encounter any problems or difficulties during the use of the model. Our team is composed of experienced scientists and technical experts who respond quickly to your needs and provide professional solutions and suggestions. We are committed to providing you with a high-quality service experience and ensuring that you can smoothly use gene-edited in vivo obesity models for scientific research.

By combining diverse in vivo models and advanced gene editing technologies, Protheragen provides clients with a comprehensive and efficient gene editing-based in vivo obesity model development service, helping researchers achieve more in-depth results in the study of obesity and related diseases. Please feel free to contact us for more details if you are interested in our gene editing-based in vivo obesity model development service!

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Chemical-induced Obesity Model
• Custom Chromosome Replacement Model Service
• Custom Multi-Gene Mutation Model Service
• Custom Single Gene Editing Model Service
• Custom Single Gene Mutation Model Service
• Custom Transgenic Model Service
• Drug Injection-based In Vivo Obesity Model Development Service
• High Fat-induced Obesity Model
• High Sugar-induced Obesity Model
• Hypothalamic Injury-based In Vivo Obesity Model Development Service
• Mammal Obesity Model
• Non-mammal Obesity Model
• Oophorectomy-based In Vivo Obesity Model Development Service
• Other Nutrient-induced Obesity Model
• Protein-induced Obesity Model