High Fat-induced Obesity Model
InquiryOverview of High Fat-induced Obesity Model
At Protheragen, we are committed to providing cutting-edge and comprehensive biotech services, including obesity and related therapeutic areas. Our services cover Preclinical Studies of Anti-Obesity Therapeutics, and go deep into the development of Obesity Models, including In Vivo Obesity Models for Obesity Research and In Vitro Obesity Models for Obesity Research. The high fat-induced obesity model is one of our core services in this field. It provides clients with an accurate and reliable experimental platform by simulating the obesity process caused by a high-fat diet.
We utilize our high fat-induced obesity model to rigorously assess the effectiveness of Anti-Obesity Therapy Development. By exposing animals to a diet rich in fats that mimic human obesogenic environments, our model recapitulates the physiological changes associated with obesity.
Harness Cutting-edge Techniques to Create High-Fat Obesity Models, Empowering Your Research to Develop Innovative Obesity Treatments and Scientific Breakthroughs
The principle of the high-fat diet-induced obesity model is to simulate the excessive energy intake caused by a long-term high-fat diet in humans by giving experimental animals a high-proportion fat diet, thereby promoting the accumulation of fat in the body and forming an obese state. This is used as an in vivo model for studying the pathological mechanism of obesity and evaluating the efficacy of anti-obesity drugs.
Model Animal Selection and Preparation
Selected model animals: C57BL/6 mice are the most widely used because they are sensitive to high-fat diets and have a clear genetic background.
Adaptive feeding: We place the model animals in a standard feeding environment for several days of adaptive feeding to ensure that their health status is stable.
High-fat Diet Formula Design and Preparation
Formula design: Design a high-fat diet formula containing a high proportion of fat and an appropriate amount of carbohydrates and protein according to research needs.
Ingredient procurement: Purchase the required ingredients, such as lard, butter, sucrose, corn starch, eggs, casein, etc.
Prepare diet: Accurately weigh the ingredients according to the formula ratio, then mix them evenly to make a high-fat feed and store them under appropriate conditions for use.
Model Construction
Animal grouping: Mice are randomly divided into a high-fat diet group and a normal diet group (control group), ensuring that there are no significant differences between the two groups of mice in terms of age, weight, gender, etc.
High-fat diet feeding: The mice in the high-fat diet group are continuously fed with a designed high-fat diet, while the control group is given a normal diet. The feeding cycle is determined according to research needs, usually for several weeks to several months.
Model Evaluation and Monitoring
Weight monitoring: Weigh the mice regularly, then record and compare the weight gain of the two groups of mice.
Biochemical index detection: Before the end of the experiment, collect blood samples from mice, and use a biochemical analyzer to detect biochemical indicators such as total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in serum.
Histopathological examination: After euthanizing the mice, key organs such as the liver and adipose tissue are quickly taken for fixation, sectioning, and staining. Pathological changes such as fatty degeneration and inflammation are observed under a microscope.
Workflow
We initiate obesity research projects by communicating with clients to clarify objectives, model needs, and timelines, followed by crafting a meticulous project plan. We then prepare and group animals, feed the high-fat diet group as per the established protocol, and conduct daily monitoring with meticulous data recording. Before experiment conclusion, we collect blood for biochemical analysis and perform histopathological examinations on tissues. Finally, we organize the data, conduct statistical analysis, compile a detailed report, and deliver it alongside raw data to clients, offering ongoing technical support and consulting services.
Applications of High Fat-induced Obesity Model
- Drug development and evaluation: The model can be used to evaluate the effects of new anti-obesity drugs.
- Nutritional research: This model can be used to help scientists study the effects of different dietary ingredients on obesity and related metabolic diseases.
- Gene and epigenetic research: The high fat-induced obesity model can be used to help researchers explore the variation, expression regulation, and epigenetic modification mechanisms of obesity-related genes.
Advantages
- Professional team and rich experience: We have a professional team of senior scientists and experimental technicians.
- Customized service: We provide customized model development services, and design experimental plans according to clients' specific needs and research purposes.
- Comprehensive technical support: We provide clients with comprehensive technical support, including experimental design consultation, data analysis guidance, and paper writing suggestions.
Publication
Technology: Construction and evaluation of the high-fat induced obesity model
Published: 2014
Journal: PLOS ONE
IF: 2.9
Results: To construct and evaluate the high-fat induced obesity model, 5-week-old male C57BL/6 mice were randomly assigned to four groups: low-fat sedentary (LF/Sed), low-fat with voluntary exercise (LF/Ex), high-fat sedentary (HF/Sed), and high-fat with voluntary exercise (HF/Ex). The diets were specifically formulated with the HF group receiving a diet composed of 60% kcal from fat. The exercise groups had access to a running wheel to record voluntary physical activity. This setup aimed to simulate diet-induced obesity by feeding the HF groups a high-fat diet while allowing HF/Ex groups access to exercise to study its effects on obesity prevention. The model's evaluation included monitoring parameters such as body weight, glucose tolerance, and gut microbiota composition using 16S rRNA gene sequencing.
Fig.1 Exercise, body weight and glucose tolerance. (Evans, et al., 2014)
Frequently Asked Questions
Can this model accurately simulate the human obesity state?
Although animal models cannot fully replicate all the complexity and diversity of human diseases, the high-fat-induced obesity model has similarities with the human obesity state in many aspects, including weight gain, fat accumulation, metabolic abnormalities, etc. We make the model closer to specific types of human obesity by fine-tuning the feed composition and feeding conditions, thereby providing a valuable reference for studying human obesity and related diseases.
What if I have questions or need to adjust a certain link in the model construction process?
We attach great importance to communication and cooperation with clients. You can contact our technical support team at any time if you have any questions or need to adjust during the model construction process. We respond to your needs in a timely manner and provide professional advice and solutions. If necessary, we also adjust the experimental plan or rebuild the model according to the specific requirements of clients to ensure the smooth progress of the research.
At Protheragen, we focus on providing a high-quality high fat-induced obesity model development service. We simulate the human obesity process through a carefully designed high-fat diet plan and accurately construct obesity models for the scientific research community. Please feel free to contact us for more details if you are interested in our fat-induced obesity model development services!
Reference
- Evans, C.C.; et al. Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity. PloS one. 2014, 9(3): e92193.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.