In Vitro Cell Models for Obesity Research
InquiryOverview of In Vitro Cell Model
The increasing prevalence of obesity has facilitated in-depth studies on the biology of adipose tissue and the precise mechanisms of adipocyte differentiation and adipogenesis. The metabolic and inflammatory disease features of obesity, such as ectopic fat deposition in key insulin-sensitive organs, inflammatory responses in white adipose tissue (AT), and infiltration of pro-inflammatory macrophages, combine to promote the development of insulin resistance, type 2 diabetes mellitus (T2D), cardiovascular disease, neurodegenerative disease, and cancer. Relying on the Obesity Model and molecular biology techniques, Protheragen provides services to analyze adipogenesis and adipocyte function in obesity. Our company provides In Vivo Obesity Models and in vitro cell models, helping clients study the process of adipogenic differentiation and its relationship with obesity and its related complications. Our goal is to be your first choice in preclinical studies of anti-obesity therapeutics.
Your Most Satisfied Partner for In Vitro Cell Models
Preliminary Analysis and Design
Advanced in vitro cell models offer a unique pathway for investigating the intricate mechanisms of adipogenic differentiation, providing insights into the pathogenesis of obesity and its comorbidities. Given their central role in energy homeostasis and metabolic health, we conduct an in-depth analysis of adipocyte phenotype and function, exploring how different environmental factors influence the fate of these cells. This enables us to tailor our cell models to replicate specific conditions, enhancing the relevance and accuracy of study outcomes.
In Vitro Cell Models
Protheragen provides a wide range of in vitro cell models for simulating and analyzing various intracellular interactions and dynamic responses. In vitro cell models play a crucial role in anti-obesity drug discovery and disease target screening. We have constructed a variety of different cell models. Importantly, if you have other in vitro cell models of interest, our dedicated team offers model customization services to meet your research needs.
Modeling
Cell line selection: According to the client's needs, our professional researchers provide you with the most suitable cell lines for obesity research. Our biological researchers have many years of mature experience in cell model construction and development.
Cell culture: We employ advanced culture techniques to ensure the viability and functionality of the cells. This includes maintaining optimal growth conditions, regular media changes, and strict monitoring of cell health.
Cell proliferation and differentiation: Depending on the research needs, our researchers induce cells to differentiate into specific cell types by adding specific inducers or changing the culture conditions. All induction processes are optimized.
Training system construction: According to different cell characteristics and uses, we provide professional services for the construction and optimization of culture systems.
Model evaluation: Our researchers observe cell morphology through a microscope to confirm cell growth status and differentiation. Our researchers detect specific molecular markers through immunofluorescence, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and other methods to verify the cell type and function.
Function testing: We test the constructed cellular models by measuring a variety of metrics. We provide physiological function tests, drug sensitivity tests, etc.
Data optimization and analysis: Based on the experimental results, we adjust and optimize the culture conditions and cell types to improve the accuracy of the model.
Professional Regulation & Analysis
Based on reliable in vitro models, our company provides simulation services of key steps in the adipogenic pathway, revealing the molecular mechanisms that regulate fat accumulation and insulin sensitivity. Our researchers analyze the role of microenvironmental changes in promoting obesity-related complications, providing important insights for the development of novel anti-obesity therapies. Moreover, we provide high-quality screening and evaluation services for potential obesity therapeutic targets.
Our researchers further analyze the response of adipocytes to various types of signaling molecules and the role of these molecules in regulating adipocyte function and metabolism. Meanwhile, for different obesity types and individual differences, our in vitro model focuses more on the evaluation of individualized treatment strategies.
Combining proteomics and metabolomics technologies, we provide comprehensive regulatory network analysis services for adipocyte differentiation, analysis of intra- and extracellular interactions, biomarker screening, and drug screening services. We also offer gene editing services for studying the function of different genes associated with adipogenesis in cells. For example, through gene silencing, we provide functional analysis of enzymes studied in inflammatory pathways, synthesis, and secretion of adipokines.
In addition, we further optimize the existing cell culture conditions to simulate the complex physiological environment in vivo, to more realistically reflect the effect of drug action and lay the foundation for clinical translation.
Workflow
Applications
- In vitro cell models can be used to analyze the molecular and cellular mechanisms underlying the development of obesity, including adipocyte differentiation, lipid accumulation, energy metabolism, and inflammatory responses associated with obesity.
- In vitro cell models can be used to screen and evaluate therapeutic drugs targeting obesity. By observing the effects of drugs on adipocyte differentiation and lipid accumulation, the therapeutic potential of drugs can be initially assessed.
- In vitro cell models can be used to analyze how adipocytes respond to different nutritional and hormonal stimuli and how these stimuli affect energy metabolism.
- In vitro cell models can be used to assess the effects of different nutrients (e.g., fatty acids, carbohydrates, etc.) on adipocyte function and provide a basis for nutritional intervention strategies.
Advantages of Us
- Our experienced cell model construction team provides customized cell model development services based on client's needs.
- Our company has many years of proven experience in cell induction and differentiation to ensure rapid and accurate induction and differentiation.
- The analytical data and research reports we provide are analyzed by a team of professional data analysts.
Learn More About Our Obesity Solutions
As a biotechnology company focusing on obesity research, we provide a full range of services from the discovery of anti-obesity targets and the development of innovative therapies to the evaluation of preclinical studies. We provide in-depth analysis of obesity causation, use cutting-edge technologies to predict obesity risk, and reveal client-individualized obesity mechanisms through the identification and analysis of obesity biomarkers. Our research is not limited to gene editing techniques to explore the potential for weight regulation, but extends to obesity microbiota analysis to delve into the complex interactions between microbes and obesity. In addition, our obesity pathobiology research reveals the biological basis behind obesity.
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Publication Data
Technology: RT-PCR, Fluorescent microscopy, Western blotting
Journal: JCI Insight
Published: 2021
IF: 6.3
Results: This study examines the metabolic and inflammatory disease features of obesity, including the accumulation of ectopic fat, white adipose tissue inflammation, and infiltration of pro-inflammatory macrophages in key insulin-sensitive organs. Studies have shown that 3T3-L1 adipocyte-derived exocysts have both positive and negative effects on the survival, proliferation, and function of pancreatic β-cells and human pancreatic islets in vitro, depending on the state of the adipocyte and the source of the adipose tissue. This study provides an important foundation for exploring the pathogenesis of metabolic diseases and developing new therapeutic approaches.
Fig.1 Effect of normal and inflammatory adipose tissue extra vesicles on INS-1E β cells. (Gesmundo, et al., 2021)
Frequently Asked Questions
What are some of the major determinants of adipocyte fate?
Major determinants of adipocyte fate include CCAAT/enhancer binding proteins (C/EBPs), peroxisome proliferator-activated receptor (PPAR-γ), and sterol regulatory element binding protein (SREBP) transcription factors.
How to characterize in vitro induction into lipid differentiation?
Our company mainly identifies cells after lipogenic induction by staining method and gene expression. Lipid droplets are stained with oil red O to give a prominent red color under the microscope, while undifferentiated cells showed no significant color. The strength of lipogenic differentiation is characterized by counting the percentage of lipogenic differentiated cells in each sample. Lipid-forming differentiation is characterized by the expression of genes characteristic of lipid formation, such as PPAR-γ. Our researchers use RT-PCR to measure the expression of these genes.
Protheragen provides various in vitro cell models for obesity research. Depending on the cell and target specificity, our model construction experts provide you with the most appropriate solution. No matter what problems you encounter in cell modeling, please contact us first!
Reference
- Gesmundo, I.; et al. Adipocyte-derived extracellular vesicles regulate the survival and function of pancreatic β cells. JCI Insight. 2021, 6(5).
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.