Overview

Adipose-derived stem cells (ADSCs) have self-renewal ability and multidirectional differentiation potential. They are easy to obtain from adipose tissue and have the advantages of low immunogenicity and high biocompatibility. Through well-designed in vitro cellular experiments and Obesity Model validation, Protheragen explores the role of ADSCs in regulating fat metabolism, promoting energy balance, and improving the inflammatory environment.

Professional ADSCs That Exceeding Your Expectations

Our company comprehensively elucidates the mechanisms of obesity and other metabolic diseases through in-depth studies of the role of ADSCs in lipid metabolism, inflammation regulation, and energy homeostasis. We provide comprehensive ADSC extraction, isolation, culture, induction, validation, and analysis services.

• Extraction, Isolation, and Culture of ADSCs

  Depending on the client's needs, our researchers select the most suitable species and adipose tissue to extract stem cells. We use collagenase digestion, centrifugation, washing, filtration, and inoculation to obtain ADSCs.

• ADSCs Surface Marker Assay

  The quality of ADSCs is ensured by flow cytometry in our lab to detect the expression of surface markers.

• Differentiation and Proliferation of ADSCs

  Our researchers have extensive experience in cell-induced differentiation. We intervene in cell growth at the most appropriate time. Our lab offers cell morphology observation, cell imaging, cell labeling, and cell staining services.

• Processing and Detection of ADSCs

  • Comet assay
  • Cell death assay
  • Quantitative polymerase chain reaction (qPCR) assay
  • Gene expression analysis

  • Micronucleus assay
  • Cell cycle
  • Flow cytometry
  • Immunofluorescence staining

Workflow

Our company provides custom ADSC modeling services according to different research needs. Our researchers provide high-quality modeling services from the acquisition of ADSCs, model construction, model validation, and obesity mechanism study to data analysis.

Applications

• ADSCs can be used to study the molecular mechanisms of obesity development, including adipocyte differentiation, lipid metabolism, and inflammatory response.
• ADSCs can be used as an in vitro model for comparing the differences in normal and obese states, revealing alterations in obesity-related genes and signaling pathways.
• ADSCs can be used for screening and evaluation of obesity-related drugs.

Advantages of Us

• The induced differentiation service provided by our company is precisely controlled according to the purpose of the experiment, effectively avoiding non-specific differentiation.
• Throughout the process, our researchers adhere to strict aseptic practices to ensure the cleanliness of the cell culture environment.
• Our company supports customized model development services to meet clients' different research needs.

Our Obesity-related Services

We have assembled a team of highly qualified experts. With years of in-depth obesity research experience and efficient operational procedures, they are dedicated to meeting the diverse research needs of our clients. With the help of cutting-edge bioanalytical technologies, our company helps clients to analyze the genetics of obesity. Our professional researchers are on standby to provide you with the most convenient and efficient services. We firmly believe that through this series of comprehensive solutions, we help clients make breakthrough progress in the field of obesity.

Publication Data

Frequently Asked Questions

What are the advantages of ADSCs?

ADSCs exhibit excellent angiogenic and immunomodulatory capabilities, as well as strong proliferative potential. Remarkably, they can be directly applied without even complex in vitro expansion steps. ADSCs have the unique ability to self-renew and differentiate in multiple directions. It can be flexibly differentiated into a variety of cell types under the appropriate induction conditions.

What are the main steps involved in isolating and culturing ADSCs?

The main steps in the isolation of ADSCs include fat washing, collagenase digestion, isolation of stromal vascular fraction, washing of stromal vascular fraction, primary inoculation, fluid exchange, and passaging culture.

Relying on a team of highly trained and talented individuals, Protheragen provides advanced ADSC models for obesity research. Our scientists want to take you deeper into obesity and explore the secrets of fat together. Please feel free to contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• 3T3-L1 Preadipocytes for Obesity Research
• Brown Adipose Cells for Obesity Research
• C2C12 Myoblasts for Obesity Research
• GT1-7 Cells for Obesity Research
• Hepatocytes (Liver Cells) for Obesity Research
• INS-1 Cells for Obesity Research
• Mesenchymal Stem Cells (MSCs) for Obesity Research
• Primary Human Adipocytes for Obesity Research
• RAW 264.7 Cells for Obesity Research
• SGBS Cells for Obesity Research
• White Fat Cells for Obesity Research