RAW 264.7 Cells for Obesity Research
InquiryOverview of RAW 264.7 Cells for Obesity Research
At Protheragen, we are committed to promoting innovation and development in the field of life sciences, especially in the research and development of anti-obesity therapeutics. As an important part of our Preclinical Studies of Anti-Obesity Therapeutics, Obesity Models provide researchers with comprehensive and in-depth obesity research solutions. Further refined to In Vitro Obesity Models for Obesity Research, we have specially launched the RAW 264.7 cells for obesity research, a scientific and technological service, which aims to use this classic mouse macrophage cell line to provide researchers with a powerful in vitro experimental platform to reveal obesity-related immune mechanisms, inflammatory responses, and drug intervention effects.
RAW 264.7 cells have unique advantages in simulating obesity-related inflammatory environments due to their strong phagocytic ability and sensitivity to immune responses. Our services not only cover basic cell culture and maintenance but also combine customized experimental designs to meet the specific needs of different research teams for exploring obesity mechanisms.
We recognize the indispensable role of RAW 264.7 cells in our Anti-Obesity Therapy Development. These macrophage cells serve as a critical model for studying inflammation and its role in obesity-related pathophysiology. By utilizing RAW 264.7 cells, we evaluate the anti-inflammatory potential of Anti-Obesity Small Molecule Drug Development and Anti-Obesity Antibody Therapy Development. This system allows us to gain insights into how these therapies mitigate obesity-induced inflammation, ultimately guiding the discovery and optimization of effective anti-obesity treatments.
Advance Your Obesity Studies with RAW 264.7 Cells
At PP Biotech, we harness the capabilities of RAW 264.7 cells, a murine macrophage model, to delve into the complexities of obesity. This versatile cell line enables us to explore various facets of obesity, including lipid metabolism, inflammation, and oxidative stress, thereby facilitating the discovery of effective therapeutic targets.
Culturing RAW 264.7 Cells
We maintain RAW 264.7 cell cultures under optimal conditions to ensure healthy growth and regularly monitor cell morphology and viability to maintain a robust cell culture.
Experimental Treatment Application
We apply specific treatments to the cells, including lipogenic agents, inflammatory stimuli, peroxisome proliferator-activated receptor (PPAR) modulators, and oxidative stressors. Well-defined controls and replicates are used to ensure the reliability and reproducibility of the results.
Assay Implementation
We conduct a variety of assays to measure the cellular responses.
Lipid Metabolism and Accumulation: We treat RAW 264.7 cells with lipogenic agents or potential anti-obesity compounds. Lipid accumulation is assessed using oil red O staining and subsequent quantification assays, providing insights into how different treatments influence lipid storage.
Inflammatory Response and Cytokine Production: RAW 264.7 cells are stimulated with agents like lipopolysaccharides (LPS) to induce an inflammatory response. We measure the secretion of key cytokines (e.g., tumor necrosis factor (TNF)-α, interleukin 6 (IL-6), IL-1β) through enzyme-linked immunosorbent assay (ELISA) and analyze gene expression changes using real-time polymerase chain reaction (qPCR) to understand the impact of various treatments on inflammation.
PPAR Pathway Activation: We transfect RAW 264.7 cells with PPAR constructs and treat them with potential agonists or antagonists. PPAR activity is measured using reporter assays, and downstream gene expression is evaluated to determine the broader metabolic effects of the treatments.
Oxidative Stress and Antioxidant Responses: RAW 264.7 cells are exposed to oxidative stressors like hydrogen peroxide to simulate oxidative damage. We then apply antioxidant treatments and assess their protective effects by measuring cell viability and oxidative stress markers.
Data Analysis
We perform statistical analyses to identify significant changes and trends. Then we interpret these findings within the broader context of obesity research to discern potential therapeutic implications.
Workflow
Applications of RAW 264.7 Cells for Obesity Research
- Study on the association between inflammation and obesity: RAW 264.7 cells are an excellent model of macrophages and can be used to simulate the inflammatory response in obesity in vitro.
- Study on the mechanism of adipose tissue remodeling: RAW 264.7 cells can be used to study the effects of macrophage polarization on adipocyte function and adipose tissue microenvironment under different conditions.
- Drug screening and evaluation: RAW 264.7 cell model provides an important platform for screening and evaluating potential drugs for obesity and its complications.
Advantages
- Easy to operate and culture: Compared with other complex cell models, RAW 264.7 cells are easy to operate and culture.
- Versatility: RAW 264.7 cells are not only suitable for inflammation and metabolism research but also widely used in research in multiple fields such as immunity, apoptosis, and tumors.
- High-fidelity model: As a classic model of macrophages, RAW 264.7 cells have high fidelity and representativeness to accurately simulate the biological behavior and function of macrophages in vivo.
Publication
Technology: Use of RAW 264.7 cells to explore the molecular link between obesity and inflammation
Published: 2014
Journal: PloS ONE
IF: 2.9
Results: The study investigates the role of phospholipase D1 (PLD1) in leptin-induced expression of the proinflammatory cytokine TNF-α in RAW 264.7 cells, suggesting a molecular link between obesity and inflammation. The researchers found that leptin enhanced PLD1 activity via activation of PLCγ and Src kinase pathways. The knockdown of PLD1 significantly reduced leptin-induced TNF-α expression and production, which indicates that PLD1 is a crucial mediator in this process. Additionally, leptin-induced PLD1 activation leads to the activation of p70S6K and JNK pathways, further driving the TNF-α production. This study highlights the importance of PLD1 in regulating inflammatory responses to leptin, thereby providing insights into the molecular mechanisms linking obesity to inflammation and respiratory issues.
Fig.1 Effect of PLD knockdown on leptin-induced TNF-α expression in Raw 264.7 cells. (Lee, et al., 2014)
Obesity Causation Analysis Services
We leverage RAW 264.7 cells, a well-established macrophage cell line, for obesity research to gain insights into the inflammatory processes underpinning obesity. Our obesity causation analysis services, which include Obesity Causation Analysis of Disease-Induced Obesity and Obesity Causation Analysis of Medication-Induced Obesity, utilize these cells to investigate the intricate mechanisms leading to obesity. These services are essential for advancing our understanding of obesity causation and for developing more effective interventions to combat this global health challenge.
Frequently Asked Questions
Why are RAW 264.7 cells an ideal choice for obesity research?
RAW 264.7 cells, as a representative of the mouse mononuclear macrophage cell line, have become an indispensable tool in obesity research due to their high functional activity and easy in vitro culture. During obesity, macrophages in adipose tissue especially the M1 type increase significantly and participate in the regulation of inflammatory response, thereby affecting the function and metabolism of adipocytes. RAW 264.7 cells can simulate this inflammatory response, helping scientists to deeply understand the complex relationship between inflammation and obesity.
How to use RAW 264.7 cells for obesity-related drug screening?
Using RAW 264.7 cells for obesity-related drug screening is a multi-step process. First, scientists design different experimental schemes according to the purpose of the research, such as simulating inflammatory response in an obese state, detecting the effect of drugs on inflammatory mediators, etc. Then, the candidate drug is added to the cell culture system to observe and record changes in cell morphology, proliferation rate, secretion of inflammatory mediators, etc. By comparing and analyzing the experimental results of different drug treatment groups, drugs with potential therapeutic effects can be preliminarily screened. Finally, the efficacy and safety of these drugs need to be further verified in animal models or preclinical studies.
Protheragen focuses on providing high-quality RAW 264.7 cells, which are key tools in obesity research. These cells help scientists to deeply explore the inflammatory mechanisms and metabolic regulation of obesity, and promote the innovation and development of treatment strategies for obesity-related diseases. Please feel free to contact us for more details if you are interested in our RAW 264.7 cells for obesity research!
Reference
- Lee, S.M.; et al. Leptin increases TNF-α expression and production through phospholipase D1 in Raw 264.7 cells. PloS one. 2014, 9(7): e102373.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.