White Fat Cells for Obesity Research
InquiryOverview of White Fat Cells
The main feature of obesity is the abnormal or excessive expansion of white adipose tissue (WAT), i.e., the increase in the size and number of fat cells. WAT is the energy reservoir in the body and is closely related to the lipid metabolism and energy balance of the whole body. At the same time, WAT is also an important endocrine organ, secreting adipokines such as adiponectin, leptin, and tumor necrosis factor-alpha (TNF-α)). Therefore, using white fat cells as an In Vitro Model to study obesity is excellent. Protheragen provides diverse Obesity Models for preclinical studies according to the different needs of our clients.
Discover the Secrets of Obesity from White Fat Cells
Adipocytes are mainly categorized into white fat cells and Brown Adipose Cells, which differ in morphology, gene expression, and function, and are transformed into each other under certain circumstances. Our company provides comprehensive white fat cell modeling, evaluation, transformation, and assay services.
Cell Culture and Induced Differentiation
Cell culture: Our researchers isolate cells from the original sample and inoculate them into culture vessels. When inoculating, attention needs to be paid to the density and distribution of the cells to ensure that the cells grow evenly.
Induced differentiation: Our researchers provide professional induced differentiation services. We have strict control over culture time, concentration, and differentiation. We select the appropriate inducer according to the target cell type. Our staff regularly observe the morphology and growth of the cells to monitor the process of induced differentiation.
Identification: We use specific staining methods or molecular biology techniques to characterize the differentiated cells. Our lab provides professional oil red O staining, boron-dipyrromethene dye (BODIPY) fluorescent staining, triglyceride content assay, reverse transcription-polymerase chain reaction (qRT-PCR), and western blot.
Our researchers collect the cells on days 0, 3, 6, 9, and 12 and observe the changes in cell size and morphology. Moreover, we further examine the production of lipid droplets, neutral lipids, and triglycerides, as well as the expression levels of genes and proteins related to adipogenesis, fatty acid-binding proteins, lipo droplet-encapsulating proteins, etc.
Study on the Mechanism of Browning of White Fat Cells
Years of research have shown that inducing the browning of WAT significantly promotes the body's energy consumption, which effectively prevents and controls obesity. Therefore, it is of great importance to study the mechanism of white fat cell browning and its inducing factors. Our company uses optimized white fat cells as a model to provide clients with adipocyte browning factors and mechanism analysis services.
Workflow
The process of white fat cell model construction and white fat cell browning mechanism research is roughly as follows: First, our researchers perform in vitro culture to establish a stable cell line model. Subsequently, the cells are intervened and induced using methods such as gene editing or drug treatment. During the browning process, we monitor the cells for morphological changes, changes in gene expression profiles, and alterations in metabolic activity. Ultimately, the molecular mechanism of white fat cell browning is studied in depth using molecular biology, biochemistry, and cell biology.
Applications
- White fat cells can be used to study the pathogenesis of obesity, anti-obesity drug screening, and preclinical outcome assessment.
- White fat cells can be used to study the pathogenesis of various metabolic diseases such as cardiovascular disease and diabetes mellitus.
- White fat cells can be used to study the effects of exposure to environmental pollutants on adipogenesis, especially during adipocyte differentiation.
Advantages of Us
- The excellent white fat cell model provided by our company highly simulates the morphology, function, and metabolic properties of white adipocytes in the human body.
- Our researchers precisely control external factors such as temperature, nutrients, and growth factors to study the effects of these factors on white fat cell function.
- Our company provides not only traditional drug induction but also gene-editing services to meet the different needs of clients in modeling.
Delve into Obesity with us!
We provide clients with comprehensive anti-obesity research programs. Our experienced experts provide you with Comprehensive Project Analysis and design and develop customized solutions for library construction, sequencing, bioinformatics analysis, microbiota research, and pathobiology research based on project characteristics.
Publication Data
Technology: Western blotting, Cytotoxicity assay, Glycerol-Glo assay
Journal: Adipocyte
Published: 2022
IF: 3.5
Results: This article focuses on exploring the potential role of oncostatin M (OSM) in regulating lipolysis in white adipocytes. The researchers used cultured white adipocytes of mouse or human origin as an in vitro model. The effect of OSM on lipolysis was assessed by assaying the activity of hormone-sensitive lipase (HSL) and other molecules associated with lipolytic mechanisms, as well as the release of free fatty acids and glycerol. The data suggest that OSM signaling directly promotes lipolysis in white adipocytes in an external signal-regulated kinase1/2-dependent manner.
Fig.1 Mapping of lipolysis data in mouse and human white adipocytes. (van Krieken, et al., 2022)
Frequently Asked Questions
What are the distribution and role of WAT respectively?
WAT is mainly distributed in the viscera and subcutis and stores excess energy in the body in the form of triglycerides. It secretes large amounts of inflammatory factors involved in the inflammatory response of the body. WAT has large fat droplets and fewer mitochondria.
What is the browning of white fat cells?
Under certain conditions, such as cold stimulation and β3 receptor activation, white fat cells are readily converted to brown fat cells. This transformation process becomes the browning of white fat cells. Exercise, diet, lifestyle habits, hormones, and intestinal flora affect the distribution and transformation of white and brown fat in the body by activating signaling pathways.
Combined with molecular biology techniques, Protheragen provides satisfactory white adipocytes for obesity studies. Our researchers provide new ideas and perspectives on the pathogenesis and drug evaluation of metabolic diseases such as obesity and diabetes. Please feel free to contact us for more information.
Reference
- van Krieken, P.P.; et al. Oncostatin M promotes lipolysis in white adipocytes. Adipocyte. 2022, 11(1): 315-324.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.