GT1-7 Cells for Obesity Research
InquiryOverview of GT1-7 Cells for Obesity Research
At Protheragen, we are committed to advancing the forefront of global anti-obesity drug research and development and accelerating the development of innovative therapies by providing comprehensive preclinical research services. As an important part of our Preclinical Studies of Anti-Obesity Therapeutics, Obesity Models bring together a variety of models that simulate the pathophysiological state of obesity to accurately evaluate the effectiveness and safety of drugs. Among them, the In Vitro Obesity Models for Obesity Research especially the GT1-7 cells for obesity research are high-end customized models tailored for scientific researchers.
As a neuronal cell line derived from the hypothalamus, the GT1-7 cell line has become an important tool for studying obesity and related metabolic diseases because it simulates physiological processes related to energy balance, feeding behavior, and metabolic regulation in the body.
We emphasize the pivotal role of GT1-7 cells in our Anti-Obesity Therapy Development. These hypothalamic neuronal cells are indispensable for studying the neural regulation of energy balance and appetite. By utilizing GT1-7 cells, we explore the mechanisms by which potential therapies modulate neuropeptide release, which is essential for Anti-Obesity Small Molecule Drug Development and Anti-Obesity Cell Therapy Development.
Harness the Power of GT1-7 Cells in Obesity Research
Protheragen offers an extensive range of scientific and technological services using GT1-7 cells for obesity research. Our expertise allows us to provide targeted and cutting-edge solutions to advance the understanding and development of anti-obesity therapies.
Mechanistic Studies
Hormonal Stimulation Studies: We study the activation of signaling pathways by hormones such as leptin and amylin. We uncover how these hormones interact and modulate energy homeostasis by analyzing the phosphorylation of key proteins, like signal transducer and activator of transcription 3 (STAT3).
Synergistic Hormonal Interactions: Our research explores how combinations of hormones affect signaling in GT1-7 cells, revealing potential synergistic effects that could inform therapeutic strategies.
Gene Expression Profiling: We conduct comprehensive gene expression analyses to identify genes regulated by obesity-related treatments. This includes examining the impact of novel anti-obesity compounds on gene expression in GT1-7 cells.
Proteomics and Protein Interaction Studies: We perform detailed studies on protein interactions and modifications within GT1-7 cells to shed light on the molecular targets affected by therapeutic interventions.
Customized Research Programs
Pathway-Specific Investigations: Our team designs and executes studies focused on specific signaling pathways in GT1-7 cells, providing insights that are crucial for the development of targeted anti-obesity therapies.
High-Throughput Compound Screening and Validation: We validate the efficacy and mechanism of action of these compounds using GT1-7 cells, ensuring reliable and actionable data for our clients.
Advanced Imaging and Analysis
Fluorescence and Confocal Microscopy: We offer advanced imaging services to visualize the subcellular localization and dynamics of proteins in GT1-7 cells. This provides critical spatial and temporal information essential for understanding obesity mechanisms.
Workflow
Applications of GT1-7 Cells for Obesity Research
- Obesity-related signaling pathway research: GT1-7 cells can be used to accurately simulate the signaling pathways in vivo that are closely related to obesity.
- Drug efficacy evaluation and screening: GT1-7 cells can be used as an ideal platform to evaluate the efficacy of anti-obesity drugs.
- Method syndrome mechanism research: We use GT1-7 cells to study how obesity affects the functions of other metabolic organs and reveal the pathogenesis of metabolic syndrome.
Advantages
- High physiological relevance: GT1-7 cells have high physiological relevance and can accurately simulate biological processes related to obesity in vivo.
- Strong experimental flexibility: Our technology platform supports a variety of experimental designs and operations, including drug treatment, gene editing, and transcriptomic analysis to meet different research needs.
- Continuous innovation and development: We keep up with the forefront of scientific research and continuously introduce new technologies to optimize the GT1-7 cell research platform to promote continuous innovation and development in the field of obesity research.
Publication
Technology: The signaling mechanisms of melanocortin-4 receptors (MC4R) in GT1-7 cells
Published: 2009
Journal: Journal of Biological Chemistry
IF: 4.0
Results: The authors discussed the use of GT1-7 cells in obesity research, particularly focused on the signaling mechanisms of MC4R in hypothalamic cells. MC4R-induced anorexigenic signaling in the hypothalamus controls body weight and energy homeostasis. The study highlights how MC4R-induced anorexigenic (appetite-reducing) signaling is crucial for body weight and energy homeostasis and has traditionally been linked to coupling with Gs proteins. However, the research reveals that even MC4R mutants deficient in Gs signaling can activate G proteins in a pertussis toxin-sensitive manner, indicating their ability to interact with Gi/o proteins.
Fig.1 Hypothalamic GT1-7 cells exclusively express the MC4R subtype. (Buech, et al., 2009)
Obesity Tissue Cell Function Analysis Service
We leverage GT1-7 cells as a pivotal asset in our obesity research, as these hypothalamic neuronal cells mimic critical aspects of central nervous system control over energy balance. They enable us to explore the neural mechanisms underlying appetite regulation and metabolic homeostasis in obesity. Our obesity tissue cell function analysis service, tightly integrated with GT1-7 cell studies, delves into the functional consequences of obesity on cellular metabolism, encompassing adipogenesis, insulin sensitivity, and lipid metabolism.
Frequently Asked Questions
What are GT1-7 cells? Why are they suitable for obesity research?
GT1-7 cells are a specially cultured and processed neuronal cell line derived from gonadotropin-releasing hormone (GnRH) neurons in the mouse hypothalamus. These cells can maintain their endocrine properties in vitro and respond to external stimuli such as overnutrition and hormone changes, simulating neuronal activity related to energy balance and appetite regulation in vivo. Therefore, GT1-7 cells are widely used in many aspects of obesity research, including but not limited to exploring the mechanism of obesity, evaluating the effects of new drugs on appetite and energy metabolism, and developing potential treatments for obesity-related complications (such as diabetes and cardiovascular disease).
How are the research results of GT1-7 cells translated into clinical applications?
The translation of GT1-7 cell research results into clinical applications is a complex and long process, which usually involves the following stages. During the translation process, our company will actively cooperate to assist clients in promoting the clinical application of GT1-7 cell research results.
- Basic Research: Delving into the intricate mechanisms of GT1-7 cells' role in obesity and associated disorders via rigorous laboratory experimentation, fostering a profound comprehension.
- Preclinical Research: Rigorously validating the efficacy and safety of our research findings in animal models, ensuring a solid foundation for subsequent human studies.
- Clinical Trials: Methodically progressing through Phases I, II, and III of clinical trials, meticulously evaluating the therapeutic potential and safety profile of drugs or treatments.
- Registration & Approval: Upon successful completion of clinical trials, submit a comprehensive registration application to the pertinent regulatory body, with the ultimate goal of securing market authorization for widespread availability.
- Marketing & Application: Leveraging innovative marketing strategies, transforming groundbreaking research outcomes into clinically accessible products and services.
Protheragen offers unparalleled expertise and customized solutions for our client's research needs. Our robust scientific and technological services using GT1-7 cells are designed to support obesity research. Please feel free to contact us for more details if you are interested in our GT1-7 cells for obesity research!
Reference
- Buech, T.R.; et al. Pertussis toxin-sensitive signaling of melanocortin-4 receptors in hypothalamic GT1-7 cells defines agouti-related protein as a biased agonist. Journal of Biological Chemistry. 2009, 284(39): 26411-26420.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.