3T3-L1 Preadipocytes for Obesity Research
InquiryOverview of 3T3-L1 Preadipocytes
Protheragen is committed to providing our clients with satisfactory Obesity Models. Our specialized researchers offer both In Vivo and In Vitro based models to meet our client's needs. The mouse embryonic fibroblast (preadipocyte) line (3T3-L1) is a continuous subline of 3T3 obtained by clonal isolation. It is particularly suitable for the study of obesity-related diseases and their dysfunctions. 3T3-L1 cells morphologically present fibroblast characteristics, but under appropriate culture conditions, they are able to differentiate into an adipocyte-like phenotype, making them a prototypical model for the study of white adipocytes.
3T3-L1 Preadipocyte-An Ideal Cell Model for Adipose Metabolism
Adipose precursor cells provide excellent in vitro cell models for studying the molecular pathways of metabolic diseases. We have experienced operators to construct reassuring 3T3-L1 preadipocyte cell models for our clients. We provide intracellular process variability analysis services according to the client's needs.
Cell Culture
Our professional researchers culture 3T3-L1 cells in vitro in appropriate media, such as Dulbecco's modified eagle medium (DMEM), adding nutrients such as serum and antibiotics to maintain cell growth and viability. In addition, we adjust the sugar and lipid content of the culture medium according to the growth characteristics of the cells to mimic the environment of lipid metabolism in vivo.
In this way, 3T3-L1 preadipocytes are able to better exhibit the characteristics of adipocytes, thus providing a strong model support for the study of diseases related to fat metabolism.
Differentiation of 3T3-L1 Preadipocytes
When the cells grow to a certain density, our researchers provide high-quality induced differentiation services. We construct 3T3-L1 preadipocyte cell models by varying the concentration and duration of action of inducers and adding new inducing drugs.
Model Validation & Analysis
Our company provides a variety of analytical services including but not limited to:
- Reactive oxygen species (ROS) measurement
- Resazurin reduction cell viability assay
- Cell viability measurement
- Real-time polymerase chain reaction (RT-PCR) assay
- Neutral red uptake cytotoxicity assay
- Caspase-3 activity measurement
- Enzyme-linked immunosorbent assay (ELISA)
- Oil red O (ORO) staining and quantification
Our researchers offer a comparison service between patients' adipose precursor cells and those of healthy donors to detect differences in intracellular processes, gene expression, and cytokine release.
Our researchers measure cytokines and provide data on inflammatory responses and insulin sensitivity by analyzing interactions between healthy adipocytes and immune cells.
We offer specialized disease target screening, diabetes drug screening, genetic screening, and biomarker screening services.
Data Integration
Our company has a professional data analysis team to collect experimental data and help clients perform statistical analysis to evaluate the reliability of the model and the effect of the intervention.
Workflow
Our professional researchers gather relevant information about the requirements, goals, and uses of cell models by communicating with clients. The collected needs are further evaluated, organized, and categorized to ensure the most satisfactory solution for the client. We provide custom cell model construction services according to the client's needs, including selecting appropriate inducers and concentrations, adjusting culture conditions, etc. Our lab evaluates 3T3-L1 preadipocyte cell models through a series of metric tests. For the series of data obtained from the analysis, our reliable data analysis team provides rapid data organization and analysis services.
Applications
- 3T3-L1 Preadipocytes can be used to study a variety of cellular and molecular mechanisms associated with insulin resistance, obesity, and diabetes.
- 3T3-L1 Preadipocytes can be used to study the physiological and pathological mechanisms controlling adipose tissue function and differentiation.
- 3T3-L1 Preadipocytes can be used as a cellular model for diabetes research or for observing the lipidogenic differentiation of mesenchymal stem cells.
Advantages of Us
- We perform strict quality control on the culture and differentiation process of 3T3-L1 cells to ensure stable cell status and reliable data.
- Our model builders have years of operational experience and extensive theoretical knowledge to ensure optimal delivery.
- Depending on the different uses for obesity, type 2 diabetes, fatty liver, and other diseases, we offer a variety of induced differentiation services and testing services.
Superior Service at Protheragen
Our company, as a front-runner in the field of obesity research, specializes in providing comprehensive solutions to obesity. Whether you are looking for targets of anti-obesity treatments, anti-obesity therapies, or pre-clinical studies on anti-obesity., our highly trained and talented team provides you with scientific solutions.
Moreover, we offer these specialized services as follows:
Publication Data
Technology: Neutral red uptake cytotoxicity assay, Resazurin reduction cell viability assay, RT-PCR assay, ELISA, Cell culture, Cell differentiation
Journal: Chemico-Biological Interactions
Published: 2021
IF: 5.1
Results: This article investigated the effect of 4-thiazolidinone derivatives on the differentiation of mouse embryonic fibroblast cell line 3T3-L1 into adipocytes. It was found that these 4-thiazolidine derivatives did not affect the adipocyte differentiation process of 3T3-L1 cells. The study demonstrated that Les-2194, Les-3377, and Les-3640 were cytotoxic to 3T3-L1 cells but did not affect cell viability or apoptotic processes. In addition, the article explored the effects of thiazolidinediones (TZDs) and their structural analogs on the differentiation of 3T3-L1 cells and found that rosiglitazone and pioglitazone at a concentration of 2 μM increased the accumulation of lipids, indicative of the hallmarks of mature adipocytes.
Fig.1 Plot of quantitative staining measurements after 14 days of differentiation. (Szychowski, et al., 2021)
Frequently Asked Questions
Why choose the 3T3-L1 cell line as a model for in vitro obesity research?
The mouse 3T3 cell line is a precursor cell line with multiple differentiation potentials and belongs to the fibroblast lineage. It has been demonstrated that the 3T3-L1 cell line is one of the most highly transformed, characterized, and stable subcellular lines in in vitro adipocyte modeling.
What biomarker analysis does Protheragen provide?
Our company provides professional biomarker analysis including but not limited to adiponectin levels, phosphorylated protein kinase B (AKT) and insulin receptor substrate-1 (IRS-1), total insulin receptor, glucose uptake, basal lipolysis, endocan, tissue inhibitor of metalloproteinase 1 (TIMP-1), insulin-like growth factor binding-protein-3 (IGFBP-3), IGFBP-6, macrophage colony-stimulating factor (M-CSF), pentraxin-3, monocyte chemoattractant protein-1 (MCP-1), resistin, Serpin family E member 1 (Serpin E1), lipocalin-2, and vascular endothelial growth factor (VEGF).
Protheragen is a leading provider of obesity models and is dedicated to advancing in vitro cell model development. Our state-of-the-art facilities are equipped with the latest analysis technologies, ensuring that our clients receive the most accurate and comprehensive data available. For any further information, do not hesitate to contact us.
Reference
- Szychowski, K.A.; et al. 4-Thiazolidinone-based derivatives do not affect differentiation of mouse embryo fibroblasts (3T3-L1 cell line) into adipocytes. Chemico-Biological Interactions. 2021, 345: 109538.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.