SGBS Cells for Obesity Research
InquiryOverview of SGBS Cells for Obesity Research
SGBS cells are a human pre-adipocyte cell strain derived from a patient with Simpson-Golabi-Behmel syndrome. Protheragen proudly provides comprehensive scientific and technological services utilizing SGBS cells for obesity research. Our services are designed to support advanced studies in obesity and related metabolic disorders by leveraging the potent and versatile SGBS cell strain. As an important part of our Preclinical Studies of Anti-Obesity Therapeutics, SGBS cells for obesity research not only enrich our Obesity Models but also one of the important contents of In Vitro Obesity Models for Obesity Research. These cells have been extensively used to study adipogenesis, the cellular mechanisms underlying obesity, and the development of anti-obesity therapeutics.
We recognize the pivotal role of SGBS cells in our anti-obesity therapy development endeavors. These cells, derived from adipose tissue, serve as an invaluable in vitro model for studying the mechanisms underlying obesity and evaluating the potential of Anti-Obesity Small Molecule Drug Development and Anti-Obesity Gene Therapy Development. By utilizing SGBS Cells, we gain deeper insights into the cellular and molecular pathways involved in obesity, facilitating the discovery and optimization of novel therapeutic strategies aimed at combating this growing health challenge.
Trust SGBS Cells for Unparalleled Insight into Adipocyte Biology and Obesity Research Advances
Cell Culture and Differentiation
Seeding: SGBS pre-adipocytes are seeded into a culture medium and allowed to reach confluence.
Induction of Differentiation: The cells are then induced to differentiate into mature adipocytes using a cocktail of differentiation-inducing agents (e.g., insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (IBMX)).
Maintenance: The cells are maintained in an appropriate adipogenic medium once differentiation is initiated.
Assessment of Differentiation
Morphological Evaluation: Differentiation is confirmed by evaluating cell morphology, primarily through oil red O staining to visualize lipid accumulation.
Gene Expression Analysis: Expression levels of adipocyte-specific markers such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and fatty acid-binding protein 4 (FABP4) are measured using quantitative polymerase chain reaction (qPCR).
Biochemical Functionality: The functionality of differentiated adipocytes is assessed by measuring enzymatic activities, such as glycerol-3-phosphate dehydrogenase (GPDH) activity, which is crucial for triglyceride synthesis.
Customization
Experimental Design: We design custom experiments based on specific research needs, ranging from evaluating the impact of various compounds on adipogenesis to studying the gene expression profiles associated with obesity.
Data Analysis and Interpretation: Our service includes comprehensive data analysis and interpretation to provide actionable insights for obesity research.
Workflow
Applications of SGBS Cells for Obesity Research
- In-depth study of the pathogenesis of obesity: SGBS cells can be used to simulate the process of adipocyte differentiation, helping researchers explore the molecular mechanism of obesity.
- Research on cell therapy: SGBS cells or their derived cell products can be used for the possibility of cell therapy, such as improving obesity by regulating adipocyte function.
- Auxiliary tools for nutritional research: SGBS cells can be used to evaluate the effects of different nutrients on adipocyte metabolism.
Advantages
- Highly simulative: SGBS cells are highly simulative and can better reflect the biological characteristics of human adipocytes.
- Easy to operate and culture: SGBS cells have strong proliferation ability and stability, and are easy to culture and operate under laboratory conditions.
- Rich metabolic pathways: SGBS cells show rich metabolic pathways during differentiation, providing rich experimental data for the study of obesity and related metabolic diseases.
Publication
Technology: Establishment of the SGBS cell model
Published: 2017
Journal: Scientific Reports
IF: 3.8
Results: The author employs SGBS pre-adipocyte cell strain as an in vitro model for obesity research, focusing on its differentiation into adipocytes and subsequent characterization. To establish the SGBS cell model, pre-adipocytes derived from the stromal vascular fraction of subcutaneous adipose tissue were cultured. These cells underwent differentiation processes, resulting in gene expression patterns indicative of both white and transient brown adipocyte phenotypes. Detailed characterization included RNA sequencing analyses and metabolic functional assays. Key characterization methods comprised measuring gene and protein expressions, particularly adipogenic markers (like PPARγ), and uncoupling protein 1 (UCP1) involved in browning. Functional assays assessed mitochondrial respiration and insulin-stimulated glucose uptake, highlighting the metabolic divergence between SGBS adipocytes and primary human white subcutaneous adipocytes (PHWSC).
Fig.1 Global transcriptome profiling of SGBS and PHWSC adipocytes. (Yeo, et al., 2017)
Obesity Tissue Cell Function Analysis Service
Our obesity tissue cell function analysis service is a critical extension of our research offerings. Through this service, we provide in-depth analysis of adipose tissue cell function, encompassing a wide range of assays to evaluate metabolic pathways, adipogenic gene expression, and insulin sensitivity, among others. Specifically, our Adipose Tissue Cell Function Analysis Service encompasses detailed RNA-Seq analysis, mitochondrial respiration measurements, and assessments of triglyceride accumulation and glucose uptake, offering a holistic view of adipocyte function and its alterations in obesity.
Frequently Asked Questions
What are SGBS cells and how are they used in obesity research?
SGBS cells are a human pre-adipocyte cell line that is widely considered to be a representative human white pre-adipocyte model. These cells can differentiate into mature adipocytes in vitro, simulating the functions and properties of human adipose tissue. In obesity research, SGBS cells are used to explore adipocyte differentiation, function, metabolic mechanisms, and associations with obesity-related diseases such as diabetes and cardiovascular disease.
What are the advantages of SGBS cells over other adipocyte models?
SGBS cells have multiple advantages. First, they come from humans, so they are closer to the actual situation in the human body, providing a more realistic model for studying obesity and related diseases. Second, SGBS cells show high induction efficiency and stability during differentiation, making experimental results more reliable. In addition, SGBS cells also have good biological characteristics, such as high insulin sensitivity and triglyceride accumulation ability, making them an ideal tool for studying the mechanism of obesity.
Protheragen focuses on providing high-quality SGBS cells. These cells are ideal models for obesity research and accurately simulate the differentiation and function of human adipocytes, helping scientists to deeply explore the pathogenesis and potential therapeutic targets of obesity. Please feel free to contact us for more details if you are interested in our SGBS cells for obesity research!
Reference
- Yeo, C.R.; et al. SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes. Scientific reports. 2017, 7(1): 4031.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.