Structural Variation in Obesity

At present, the most studied single genes and common obesity are mainly single nucleotide variations, and the role of copy number variation (CNV) in obesity is poorly understood. CNVs are DNA fragments with different diploid copy numbers in different individuals and are divided into common variants (frequency greater than 5%) and rare variants (frequency less than 1%). Studies have found that CNVs on chromosomes 10q11.22 (PPYR1 gene) and 11q11 (olfactory receptor gene) are associated with increased body mass index (BMI) and early-onset extreme obesity. Furthermore, meta-analyses link common CNVs within NEGR1 and near GPRC5B to body weight regulation. Large CNVs are associated with significantly increased risks of severe obesity, autism, and schizophrenia, while reciprocal duplications of this locus are associated with underweight and smaller head circumference. Protheragen develops one-stop obesity research solutions and is committed to meeting any needs of clients in obesity research. We not only provide services such as obesity gene editing and Obesity Microbiota Research but also develop anti-obesity therapies targeting specific targets.

Precision Solutions for Health: Targeted Editing of Obesity Genes for a Fit Future

Understanding the genetic basis of obesity provides new insights into its prevention and treatment development. Targeted gene editing provides an innovative way to modify genes associated with obesity, which has the potential to mitigate its effects. Protheragen combines expertise and technology in the field of obesity and genomics to provide clients with advanced obesity candidate gene screening services and obesity gene editing services (knock-in, knock-out, overexpression, silencing, etc.). Our services are as follows:

• Candidate Obesity Gene Screening Service

Many obesity candidate gene studies have identified at least 127 genes associated with obesity or related phenotypes, such as MC4R, BDNF, PCSK1, ADRB3, and PPARγ. Although there are some conflicting findings due to differences in study parameters, these studies are still valuable for understanding the genetic influence on obesity. We use bioinformatics and transcriptome sequencing tools to identify potential obesity-related genes in vitro and obtain a comprehensive view of gene expression profiles in various tissues. In addition, we use the Drosophila system to validate candidate genes in vivo and observe the effects of genetic alterations on metabolic processes and fat storage.



Bioinformatics-based Obesity Gene Screening Service



Transcriptome Sequencing-based Obesity Gene Screening Service



Drosophila Systematic Functional Screening Service

• Obesity Gene Editing Service

Our obesity gene editing service aims to address the complexity of genetic factors that cause obesity through comprehensive and precise gene editing technology. We provide obesity-related single-gene and multi-gene editing services using gene editing technologies to ensure that obesity-related genes are targeted with high precision and efficiency. Our goal is to enable researchers to gain a deeper understanding of the genetic mechanisms behind obesity and pave the way for innovative therapeutic interventions.

• Obesity Single Gene Editing Service: The obesity single gene editing service is designed to target and modify specific genes associated with obesity, thereby addressing the root causes of obesity at the molecular level. By editing genes that regulate fat storage, metabolism, and appetite, it provides a personalized weight management approach that goes beyond traditional diet and exercise methods. The potential benefits of this approach are improved metabolic health, reduced risk of obesity-related diseases, and enhanced overall well-being.
• Obesity Multigene Gene Editing Service: Unlike single-gene editing, obesity multi-gene editing services target multiple genes known to affect body weight and metabolism. We use advanced gene editing technologies to carefully edit multiple gene sites simultaneously to optimize metabolic pathways, reduce fat accumulation, and improve energy expenditure. This approach not only provides a more powerful and permanent solution to obesity but also minimizes the possibility of compensatory mechanisms that typically undermine single gene therapy.
• Strategies for Obesity Gene Editing: We offer a full suite of obesity gene editing services, including knock-in, knock-out, overexpression, and gene silencing, each carefully executed to target specific obesity genes and obesity-related pathways. Our knock-in services allow us to insert beneficial genes to enhance metabolic function, while our knock-out capabilities allow us to disable dysregulated genes that contribute to obesity. Our overexpression protocols amplify the activity of genes that naturally help regulate body weight, while our silencing services effectively reduce the activity of genes that promote fat accumulation. By leveraging these state-of-the-art genetic tools, we enable researchers to develop targeted treatments and interventions to address obesity at its genetic root causes.

  

  Gene Knockout

  

  Gene Overexpression

  

  Gene Silencing

  

  Gene Knockin

Applications

• Mechanistic research: Understanding the specific roles and mechanisms of single and multiple obesity-related genes.
• Therapeutic development: Identifying and validating new therapeutic targets for obesity treatment.
• Functional genomics: Exploring gene functions and interaction networks associated with obesity and metabolic disorders.
• Drug screening: Creating gene-edited cell and animal models for high-throughput drug screening.
• Nutritional genomics: Studying the interaction between dietary factors and genetic predisposition to obesity.

Advantages

• High precision: Using cutting-edge gene editing technologies ensures high specificity and minimal off-target effects.
• Versatility: Our services include a range of editing strategies, such as knock-in, knock-out, overexpression, and gene silencing, to meet a variety of research needs.
• Customized solutions: Tailored editing plans are designed to meet the unique requirements of each research project.
• Comprehensive support: From experimental design to data analysis, our team provides comprehensive support throughout the gene editing process.

Publication Data

Frequently Asked Questions

What technology do you use for obesity gene editing?

We use the most advanced gene editing technologies and transcription activator-like effector nuclease (TALEN) to precisely alter obesity-related genes. These advanced tools enable us to perform knock-in, knock-out, overexpression, and silencing strategies with high specificity and minimal off-target effects, ensuring the reliability and effectiveness of our targeted gene editing interventions.

Do you provide support and consultation throughout the gene-editing process?

Yes, we provide comprehensive support and consultation throughout the entire gene editing process. Our team of genetic experts works closely with you from the initial project design and planning stages to ensure that goals and methods are aligned with your research objectives. We provide ongoing updates on the progress of gene editing procedures and address any questions or concerns you may have along the way. Our post-editing support includes data analysis, interpretation of results, and advice on the next steps, ensuring you have a seamless and informed experience from start to finish.

At Protheragen, we have unparalleled expertise in targeted gene editing, offering knock-in, knock-out, overexpression, and gene silencing of obesity genes. Whether you need to manipulate a single gene or the interaction between multiple genes, our customized strategies ensure accurate and efficient results. Please feel free to contact us to discuss how our cutting-edge services meet your specific research needs.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Diagnostic Tests
• Obesity Biomarker Identification and Analysis Service
• Obesity Causation Analysis Services
• Obesity Microbiota Research Service
• Obesity Pathobiology Research Service
• Obesity Prediction Service
• Weight Loss and Weight Management