Overview

Obesity is a multifactorial, relapsing, chronic disease. The continuous development of genetic engineering techniques and tools, as well as a deeper understanding of the molecular mechanisms underlying obesity and its metabolic complications, has fuelled the development of anti-obesity therapies based on adipose tissue and stem cells. It holds great promise for applications in regenerative medicine and addressing a wide range of medical needs. Protheragen is at the forefront of anti-obesity cell therapies and offers a customized stem cell therapy development service based on rigorous research and innovative approaches. We offer a full range of experimental support to help advance research into effective therapies for the prevention and treatment of obesity and its complications.

Working with Us to Promote Your Success in Anti-Obesity Stem Cell Therapy Research

Anti-Obesity Stem Cell Therapy Development

Stem cell-based anti-obesity therapies are promising tools for the treatment and intervention of obesity. We offer services including Cell Therapy research and related gene editing.

Development of mesenchymal stem cell (MCS)-based anti-obesity cell therapy: MSCs are a promising tool for cell therapy due to their ease of abundance and isolation. We offer a personalized MSC-based cell therapy development service, including the following, and provide full support for client research.

• Adipose tissue-derived mesenchymal stem cell (ADMSC): ADMSC has several advantages including strong differentiation into other cell types and expansion, immunomodulatory properties, easiest access, the release of multiple bioactive molecules, and angiogenic factors. Transplantation of ADMSC is found to reverse hepatic steatosis, restore glucose homeostasis by promoting insulin production and improving insulin sensitivity, and reduce adipose tissue inflammation. ADMSC-mediated gene therapy is also a safe and promising therapeutic strategy to combat the effects of obesity-induced hyperglycemia. We genetically modify ADMSC or directly transplanted ADMSC into the obese animal model and examine changes in physiological status to evaluate the therapeutic potential and safety of this therapy.
• Human umbilical cord-derived mesenchymal stromal cells (HUCMSCs): HUCMSC is one of the sources of MSC with several advantages. Overexpression of interleukin10 in HUCMSC is found to be a potential strategy for preventing obesity by counteracting obesity-related metabolic symptoms induced by a high-fat diet (HFD). We establish genetically modified HUCMSCs and inject them into animal models to study their effects on obesity and metabolic disorders.

Development of anti-obesity cell therapy based on induced pluripotent stem cell (hiPSC): HiPSC differentiates into brown or white adipose progenitor cells, providing a rich source for studying adipocyte development and showing great potential for obesity therapy. We offer custom gene editing of hiPSC, differentiation, and development of hiPSC-based anti-obesity cells.

Preclinical Studies of Therapies

We construct relevant Obesity Models (high-fat diet-induced Mice, Rat models, etc.) and transplant stem cells that are genetically modified into obese animal models. The therapeutic potential and safety of stem cell therapy are evaluated by analyzing obesity-related metabolic symptoms such as insulin resistance, glucose tolerance, lipid accumulation in the liver and adipocytes, and signaling pathways in animal models. The evaluation of the efficacy and safety of stem cell therapy includes the following:

We use several strategies to modify the gene expression profile of stem cells, including viral and non-viral-based transfection and other gene editing techniques. Stem cells are genetically modified to enhance their proliferation, viability, immunomodulation, and other important properties to increase their therapeutic potential and improve adverse effects after transplantation.

• Virus-based transfection: Viral vectors are the most commonly used tools for modifying the genome of stem cells, with a higher efficiency of DNA transfer. This method also ensures long-term and stable transcription of the target genes without altering the cell immunophenotypic characteristics or differentiation capacity, which is highly efficient. Adenoviruses and lentiviruses are the main viral vectors used for transduction.
• Non-viral methods: This method has been used to enhance the differentiation and proliferation of ADMSC. We perform genetic modifications by physical processes (e.g. nuclear transfection, electroporation, and sonoporation) or chemical methods (including inorganic nanoparticles, lipid agents, and polymers).
• Other gene editing techniques: We have specific and efficient gene editing technologies to effectively manipulate gene expression in stem cells and analyze changes related to therapeutic effects through animal models.

Workflow

The design and conduct of all experimental protocols in the development of stem cell therapies are tailored to the client's research objectives and specific needs.

Applications

• Obesity is associated with impaired glucose tolerance and often leads to type 2 diabetes. The development of new anti-obesity stem cell therapy strategies helps provide valuable information for treating diabetes and effectively reversing long-term complications.
• Nonalcoholic fatty liver disease (NAFLD): NAFLD is a disease caused by excessive fat accumulation in liver cells in the form of triglycerides. Anti-obesity stem cell therapeutic strategies may be a promising approach for potential treatment of NAFLD.
• Combination therapy development: The development of stem cell therapeutic strategies supports the design and study of combination therapies.

Advantages

• We provide reliable technical support to our clients with a team of scientists specializing in anti-obesity and stem cell therapy research with rich experimental expertise.
• As a reliable and trustworthy partner in the field of anti-obesity research, we carefully design experimental protocols and update research progress in real-time.
• Our lab team is highly skilled in performing experiments for stem cell therapy development efficiently and accurately, producing high-quality research results.

Other Personalized Services We Provide

Our experienced research team is at the forefront of obesity research and is committed to providing flexible and innovative solutions to support our client's needs in all aspects of obesity research. Our services are not limited to the development and evaluation of anti-obesity stem cell therapies but also include a comprehensive analysis of obesity risk, precise diagnostic tests, obesity prediction services, gene editing for a variety of research purposes, sophisticated testing of obesity-related biomarkers, in-depth microbiome profiling, and in-depth pathology studies. Further, with our in-depth experience, we also offer a customized weight loss and weight management service.

Publication Data

Frequently Asked Questions

What are the advantages of stem cell therapy?

Stem cell therapy is a promising and versatile option with multiple benefits, including tissue regeneration, reduction of inflammation, etc. Regenerative medicine, with stem cell therapy at its core, plays a role in the treatment of major diseases of the nervous and hematological systems and organs such as the liver, kidney, and pancreas. Among them, MSC has shown amazing therapeutic potential for obesity diseases, neurodegenerative diseases, immune disorders, diabetes, and others.

What are the advantages of MSC in the development of anti-obesity cell therapies?

As a pluripotent stromal cell, MSCs can self-renew and differentiate into multiple cell types. They have a variety of properties, such as immunomodulatory properties, the ability to migrate to a wide range of tissues, and the potential to differentiate into multiple lineages. Based on these properties, MSC has become a promising strategy for the treatment of obesity.

Based on our understanding of the molecular mechanisms of obesity and our extensive research experience, Protheragen offers an innovative and flexible service for the development of anti-obesity stem cell therapies. Our solutions are a good choice to accelerate your anti-obesity research. Please feel free to contact us for more detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• CAR-T Cell Therapy Development