C2C12 Myoblasts for Obesity Research
InquiryOverview of C2C12 Myoblasts for Obesity Research
At Protheragen, we are committed to promoting innovation and development in the field of biotechnology, especially in the fight against global health challenges such as obesity. As an important part of our Preclinical Studies of Anti-Obesity Therapeutics, Obesity Models provide researchers with a variety of in vitro and in vivo models to further explore the pathogenesis and potential treatments of obesity. Among them, In Vitro Obesity Models for Obesity Research focuses on using C2C12 myoblasts as a unique in vitro research platform, opening up a new perspective for obesity research.
C2C12 myoblasts is a rapidly differentiating myoblast cell line widely used in muscle biology and regenerative medicine. Through specific culture conditions and induction strategies, C2C12 cells simulate obesity-related metabolic changes, making them an ideal model for studying the pathophysiological mechanisms of obesity and drug screening.
We highlight the significant role of C2C12 myoblasts in our Anti-Obesity Therapy Development. These muscle precursor cells are instrumental in understanding muscle metabolism and its interplay with obesity. By employing C2C12 myoblasts, we investigate the impact of potential therapies on muscle insulin sensitivity and energy expenditure, which are crucial for Anti-Obesity Small Molecule Drug Development and Anti-Obesity Gene Therapy Development.
Revolutionize Your Obesity Research with Our C2C12 Myoblasts
C2C12 myoblasts are a widely used cell line derived from mouse skeletal muscle cells, routinely utilized to study muscle biology, differentiation, and metabolic functions. Their application in obesity research is multifaceted and invaluable for gaining insights into muscle metabolism, insulin resistance, and lipid accumulation associated with obesity.
Utilization in Obesity Research
Insulin Resistance Studies: We employ C2C12 myoblasts to explore the intricacies of insulin signaling pathways. We replicate insulin resistance by cultivating these cells under hyperglycemic and hyperlipidemic conditions. This allows us to dissect the pathways involved and identify potential therapeutic targets.
Lipid Accumulation and Metabolism: We observe their lipid metabolism by subjecting C2C12 cells to varying concentrations of glucose and fatty acids. These experiments shed light on how muscle cells manage lipid storage and mobilization under obese conditions, providing insights into the metabolic shifts associated with obesity.
Mitochondrial Function: We assess how obesity-inducing conditions impact mitochondrial health and function within C2C12 myoblasts. Understanding mitochondrial dynamics and oxidative stress is crucial for comprehending the energetic disruptions in muscle cells in the context of obesity.
Research Steps and Operation
Cell Culturing: Our process begins with culturing C2C12 myoblasts in mediums simulating physiological glucose levels. We then gradually introduce hyperglycemic and hyperlipidemic conditions to create cellular environments akin to those found in obesity.
Experimental Treatments: We administer precise concentrations of glucose and fatty acids such as bovine serum albumin-conjugated palmitic acid to the cultured cells. These treatments help us simulate the varied metabolic states observed in obesity.
Analytical Measurements: We measure mitochondrial fragmentation, membrane potential, and reactive oxygen species (ROS) production using advanced techniques like fluorescence microscopy and flow cytometry. These metrics are vital for understanding cellular responses to our experimental conditions.
Workflow
Applications of C2C12 Myoblasts for Obesity Research
- Simulating obesity-related metabolic environments: C2C12 myoblasts can be used to simulate the response of human skeletal muscle cells to obesity-related metabolic changes in vitro.
- Evaluating the effects of weight loss drugs: C2C12 myoblasts can be used to quickly screen and evaluate the effects of potential weight loss drugs.
- Studying the intervention effects of exercise on obesity: C2C12 myoblasts can be used to simulate the effects of exercise on muscle cells.
Advantages
- High controllability: C2C12 myoblasts have high controllability under in vitro culture conditions, and the culture environment can be easily adjusted to simulate different physiological and pathological states.
- Rapid responsiveness: C2C12 myoblasts have a fast response speed to environmental changes, reflecting changes in cell metabolism and morphology in a short time.
- Wide applicability: C2C12 myoblasts are not only suitable for obesity research but also can be widely used in muscle development, disease model establishment, drug screening, and other fields.
Publication
Technology: Investigate the effects of plasma from obese (OB) and obese diabetic (OBD) patients on C2C12 myoblast differentiation and bioenergetics
Published: 2021
Journal: Biomolecules
IF: 4.8
Results: The study explores the impact of plasma from OB and OBD patients on the differentiation and bioenergetics of C2C12 myoblasts, a mouse muscle cell line. Plasma exposure from both OB and OBD patients led to decreased expression of genes implicated in skeletal muscle differentiation and metabolism, such as Atgl and Pgc1b, with an additional upregulation of miR-378a-3p in OBD-treated cells. This exposure affected the cells' morphology, leading to impaired differentiation, and altered mitochondrial function. Specifically, OBD plasma reduced the maximal mitochondrial respiration rate, while OB plasma induced a metabolic shift toward glycolysis. The findings highlight the disease-specific regulatory actions of OB and OBD plasma on muscle cell function, potentially contributing to the metabolic dysfunctions observed in these conditions.
Fig.1 Study design. (Khromova, et al., 2021)
Obesity Tissue Cell Function Analysis Service
Our obesity tissue cell function analysis service builds upon this foundation by offering comprehensive analyses of the functional changes in obesity-affected tissues. This service encompasses obesity-related metabolic dysfunction analysis, which delves into the disturbances in glucose metabolism, insulin sensitivity, and lipid handling that occur in obesity. These services are vital for unraveling the complex interplay between muscle and adipose tissues in obesity and guiding the development of targeted therapies to improve metabolic health.
Frequently Asked Questions
What can C2C12 myoblasts do specifically in obesity research?
C2C12 myoblasts can simulate the functions and responses of human skeletal muscle cells in vitro, helping scientists gain a deeper understanding of the metabolic changes in muscle cells under obese conditions. By adjusting culture conditions, such as increasing the concentration of nutrients such as glucose and fatty acids, we observe the metabolic behavior of C2C12 cells in a simulated obesity environment, such as fatty acid oxidation, insulin sensitivity, etc., thereby providing a scientific basis for the treatment of obesity and its complications.
Why choose C2C12 myoblasts for obesity research instead of other types of cells?
There are several main reasons why C2C12 myoblasts are an ideal choice for obesity research.
- C2C12 myoblasts have high proliferation and differentiation potential, and can rapidly proliferate and differentiate into mature skeletal muscle cells in vitro.
- C2C12 myoblasts are sensitive to physiological and pathological stimuli and can accurately reflect the metabolic changes of muscle cells under obese conditions.
- C2C12 myoblasts culture technology is relatively mature, easy to operate, low cost, and suitable for large-scale research and long-term experiments.
At Protheragen, our C2C12 myoblasts provide a powerful cell model for obesity research, helping scientists to further explore the mechanisms of obesity in vitro, evaluate the effects of weight loss drugs, and develop personalized treatment plans. Please feel free to contact us for more details if you are interested in our C2C12 myoblasts for obesity research!
Reference
- Khromova, N.V.; et al. Regulatory action of plasma from patients with obesity and diabetes towards muscle cells differentiation and bioenergetics revealed by the C2C12 cell model and microRNA analysis. Biomolecules. 2021, 11(6): 769.
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