Overview

Obesity is triggered by a variety of factors, such as genetics, environmental factors, and so on. It is crucial to develop anti-obesity therapies against specific targets or safer drugs with superior efficacy. Animal models (single gene mutation models, etc.) are reliable tools to understand the physiological and genetic basis of obesity and to evaluate the safety and therapeutic efficacy of various therapies. Analysis of changes in their body weight, physiological characteristics, gene expression, etc., not only helps to discover and validate new therapeutic targets but also provides valuable information for the optimization of anti-obesity therapies. Based on our rich experience in obesity modeling, Protheragen provides a high-quality custom single gene mutation obesity model service. We understand the specificity of each project and carefully design the experimental process to best meet the needs of our clients.

High-quality Custom Single Gene Mutation Modelling Service Rely on Talented Team

We are the optimal partner for obesity modeling. Our professional attitude and cutting-edge technology make us a leader in this field. Specific mutation models and services include the following:

• Single Gene Mutation Models

  A number of single gene mutation models have been developed, such as obese-hyperglycemic (ob/ob) mouse, diabetic-obese (db/db) mouse, Zucker diabetic fatty (ZDF) rats, and so on. These genetic obesity models have defects in the leptin signaling pathway, and exhibit obesity characteristics and sustained weight gain within weeks after birth, making them suitable for subsequent validation of anti-obesity therapeutic targets and treatments. In addition to this, we also customize various single gene mutation models and validate the models through a variety of analytical techniques.

  Model construction: We combine various means, including chemically induced gene capture, prokaryotic injection, lentiviral vector-based transduction, etc., to construct various single-gene mutation models. Proficiency in a variety of techniques allows us to customize various single gene mutation models in the fastest possible time.

  Model validation: After the models are constructed, we validate these models through whole genome sequencing, genotyping, phenotypic analysis, pathological changes, etc. Through the validation of model accuracy, we ensure that single gene mutation models accurately meet the research needs of obesity and various therapeutic approaches.

• Anti-Obesity Therapy Development

  We utilize a variety of single gene mutation models to develop anti-obesity therapies and evaluate their efficacy and safety. Based on comprehensive experimental techniques and years of experience in preclinical studies of anti-obesity drugs, our research team analyses the therapeutic effects and mechanisms of action of anti-obesity drugs. We provide a deeper understanding and mine useful information to help advance client research.

  Anti-Obesity Small Molecule Drug Development

  Anti-Obesity Gene Therapy Development

  Anti-Obesity Cell Therapy Development

  Anti-Obesity Antibody Therapy Development

  Anti-Obesity Tissue Engineering Therapy Development

  Anti-Obesity Nanotherapy Development

Workflow

Applications

• Pathogenesis of obesity: The single gene mutation model is an ideal model to explore the pathogenesis of obesity and the influence of genes and environmental factors on obesity.
• Anti-obesity therapies: The single gene mutation model is used to study the effects and safety of various obesity-related drugs and anti-obesity therapies.
• Obesity complications: The single gene mutation model is used to study the pathogenesis of obesity complications such as type 2 diabetes and metabolic syndrome, and to explore the relationship between obesity, insulin resistance, and metabolic disorders.

Advantages

• We customize single gene mutation models to accelerate obesity pathogenesis and pharmacodynamic validation of treatments.
• Our research team is composed of experts with many years of experience in animal model research and provides a one-stop model construction service.
• We have a comprehensive quality management system to ensure that the entire obesity model construction and validation process is carried out efficiently and accurately.

Other Cutting-edge Services

We have an experienced research team that remains at the forefront of obesity research and is committed to providing reliable services to support the study of obesity mechanisms and the development of anti-obesity therapies. In addition to obesity modeling, our team integrates multiple factors to comprehensively assess obesity risk. We also provide obesity prediction services through high-precision diagnostic tests. Various advanced research equipment and strict experimental standards ensure accurate data. We also conduct in-depth analyses of obesity-related biomarkers, microbiomes, and pathologies to study obesity. Our team also develops personalized weight loss plans and weight management programs.

Publication Data

Frequently Asked Questions

What experiments do we use to validate customized single gene mutation models?

We conduct a series of biological experiments to validate the accuracy and reliability of the models. These experiments include whole genome sequencing, histopathological analyses, determination of relevant biomarkers, gene expression profiling, and so on. Through these experimental validations, we ensure that the single-gene mutation models we provide accurately reflect the relevant physiological characteristics and provide strong support to our clients.

What single gene mutation models have been developed for obesity research?

• ob/ob Mouse: The ob/ob mouse is a genetic mouse model widely used to study obesity and type 2 diabetes. These mice carry mutations in the ob gene that result in leptin deficiency and exhibit severe obesity, insulin resistance, and other symptoms.
• db/db Mouse: The db/db mouse is a spontaneous type 2 diabetes model that is routinely used for obesity research and evaluation of anti-obesity therapies. Among them are leptin receptor-deficient, prone to obesity, rapid weight gain, reduced activity, and high blood lipids. Glucose and insulin increases are insignificant at a young age. Glucose and insulin levels rise later in life. Pure db/db mice are polyphagic, polydipsic, polyuric, and sterile.
• ZDF rats: ZDF rats possess a variety of characteristics, including obesity, insulin resistance, abnormal glucose tolerance test, hyperinsulinemia, hyperleptinemia, hyperlipidemia, etc.

At Protheragen, our animal model construction technology is highly sophisticated and we offer a one-stop service for customized single gene mutation models. We help accelerate the discovery of obesity therapeutics by creating obesity models with specific mutations. Please feel free to contact us for more details on the customization of mutation models if this is of interest to you.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Chemical-induced Obesity Model
• Custom Chromosome Replacement Model Service
• Custom Multi-Gene Mutation Model Service
• Custom Single Gene Editing Model Service
• Custom Transgenic Model Service
• Drug Injection-based In Vivo Obesity Model Development Service
• Gene Editing-based In Vivo Obesity Model Development Service
• High Fat-induced Obesity Model
• High Sugar-induced Obesity Model
• Hypothalamic Injury-based In Vivo Obesity Model Development Service
• Mammal Obesity Model
• Non-mammal Obesity Model
• Oophorectomy-based In Vivo Obesity Model Development Service
• Other Nutrient-induced Obesity Model
• Protein-induced Obesity Model