Other Nutrient-induced Obesity Model
InquiryOverview of Other Nutrient-induced Obesity Model
At Protheragen, we are committed to providing comprehensive and customized biotech services, especially in the field of obesity research. With the increasing incidence of obesity worldwide, the development of effective anti-obesity drugs has become an urgent need in the current medical field. As an important part of our Preclinical Studies of Anti-Obesity Therapeutics service, we launch the other nutrient-induced obesity model construction and evaluation service. This service innovatively expands the focus to the obesity mechanism induced by non-traditional nutritional factors such as vitamins, minerals, and amino acids, providing researchers and pharmaceutical companies with a more comprehensive and in-depth selection of obesity models.
Through our Obesity Models service, we accumulate rich experience in animal model construction especially in In Vivo Obesity Models for Obesity Research. As one of our featured services, the other nutrient-induced obesity model aims to provide an important tool for revealing the complex mechanism of obesity and evaluating the efficacy of anti-obesity drugs by simulating the obesity phenomenon caused by excessive intake of specific nutritional factors.
We employ our other nutrient-induced obesity model as a valuable asset in advancing Anti-Obesity Therapy Development. This model provides insights into the complex interplay between specific nutrients and obesity pathogenesis, enabling us to evaluate the effectiveness of potential therapies under diverse dietary conditions.
Optimize Obesity Research with Our Specialized Nutrient-induced Models, Unlocking Pathways to Healthier Futures and Innovative Treatments
Traditional nutrient-induced obesity models generally focus on diets high in fats and sugars. However, emerging research indicates that overconsumption or imbalance of other nutrients such as vitamins, minerals, and specific amino acids also contribute to obesity. Our other nutrient-induced obesity model provides a unique platform to study obesity driven by non-traditional dietary factors.
Model Animal Selection
When constructing the other nutrient-induced obesity model, we usually choose C57BL/6 mice as model animals because they are sensitive to various dietary changes and easy to raise. We also select other suitable model animals according to client needs and specific research purposes.
Nutritional Factor Selection and Ratio Setting
Nutritional factor selection: We focus on non-traditional nutritional factors such as vitamins such as excessive vitamin D and vitamin A, minerals such as calcium, iron, and zinc imbalance, amino acids such as excessive branched-chain amino acids, etc. These factors are beneficial to the human body when taken in moderation but excessive intake may induce obesity.
Ratio setting: By consulting a large number of literature and pre-experiments, we accurately set the ratio of these nutritional factors in the diet to ensure that the obese phenotype is induced stably and reliably.
Model Construction
Diet formula design: We design a special feed formula based on the selected nutritional factors and ratios.
Animal feeding: We randomly divide the model animals into a control group and an experimental group, and give them standard feed and special feed respectively.
Weight monitoring: We regularly record the weight changes of animals to evaluate the effect of obesity induction.
Biochemical index detection: We collect blood samples, test biochemical indexes such as blood sugar, blood lipids, and insulin, and evaluate metabolic status.
Histopathological examination: We perform histopathological examinations on key organs such as the liver and adipose tissue to observe fat accumulation and cell morphological changes.
Model Evaluation
We quantitatively evaluate the effect of obesity induction through weight growth curves, body fat ratio, biochemical indexes, and other data.
We qualitatively evaluate the impact of obesity on organ structure and function combined with histopathological examinations.
If necessary, we conduct in-depth studies such as gene expression profiling and metabolomics to explore the molecular mechanism of obesity.
Workflow
We engage in in-depth discussions with clients to clarify research objectives and tailor nutrient-induced obesity models. We design construction plans, collaborating with feed manufacturers to prepare specialized diets. Model animals are fed and monitored for weight changes, undergoing biochemical and pathological testing at key intervals. Collected data undergoes rigorous analysis, culminating in a detailed research report. We then present results to clients, offering follow-up services based on their feedback.
Applications of Other Nutrient-induced Obesity Model
- Drug development and evaluation: This model can be used to test the therapeutic effect and safety of new drugs in obesity and related metabolic diseases.
- Nutritional intervention research: This model can be used to evaluate the preventive and therapeutic effects of different dietary interventions on obesity and its complications.
- Pathological mechanism research: Comparison of physiological and biochemical indicators and tissue pathological changes between normal mice and obese model mice helps explore the pathogenesis of obesity further.
Advantages
- Professional team and rich experience: We have a research and development team composed of senior scientists and professional technicians with rich experience in developing nutrition-induced obesity models.
- One-stop service: In addition to model development, we also provide a series of follow-up services such as model feeding, data collection, and pathological analysis.
- Continuous technical support: We provide clients with full technical support and consulting services during the development and use of the model.
Publication
Published: 2022
Journal: Nutrients
IF: 4.8
Results: The authors summarized several methods for regulating amino acid levels to induce obesity models. First, they pointed out that excessive intake of certain amino acids such as leucine may promoted fat storage and weight gain. Second, amino acid deficiency may lead to metabolic disorders and decreased insulin sensitivity, which in turn affects body weight and fat storage. Third, appropriate supplementation of specific amino acids improves insulin sensitivity and fat metabolism, which helps to reduce obesity. Maintaining amino acid balance is essential for weight regulation, so adjusting amino acid levels to keep them balanced is an important strategy for preventing or treating obesity.
Fig.1 Activation of mammalian target of rapamycin complex 1 (mTORC1) by leucine in muscle cells. (De Bandt, et al., 2022)
Frequently Asked Questions
Which clients are your company's other nutrient-induced obesity model development services mainly for?
Our services are mainly for scientific research institutions (such as universities and research institutes), pharmaceutical companies, nutrition and health product developers, and medical institutions that focus on obesity-related disease research. These clients hope to establish a specific nutrient-induced obesity model to deeply study the pathophysiological mechanism of obesity and evaluate the effectiveness and safety of weight loss drugs, functional foods, or nutritional intervention strategies.
How does your company protect clients' experimental data and research results?
We are well aware of the importance of clients' experimental data and research results, so we have taken strict data protection measures. All experimental data will be encrypted and stored, and access is limited to project team members within the scope of authorization. We sign a confidentiality agreement with clients to clarify the rights and obligations of both parties and ensure that clients' intellectual property rights are fully protected. In addition, we will back up data regularly to prevent accidental loss or damage.
At Protheragen, we focus on providing comprehensive other nutrient-induced obesity model development services. We are committed to simulating and studying obesity models induced by specific nutrients, providing scientific and reliable animal model platforms for research institutions and pharmaceutical companies to explore the mechanisms of obesity and evaluate the efficacy of weight loss drugs and functional foods. Please feel free to contact us for more details if you are interested in our other nutrient-induced obesity model development services!
Reference
- De Bandt, J.P.; et al. Branched-chain amino acids and insulin resistance, from protein supply to diet-induced obesity. Nutrients. 2022, 15(1): 68.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.