Overview

Obesity is a chronic metabolic disease caused by excessive calorie intake or underconsumption, resulting in excessive fat and abnormal distribution. It increases the risk of coronary heart disease, type 2 diabetes mellitus, hypertension, and certain tumors. Prevention and treatment of obesity are particularly important. The establishment of a good animal model of obesity is a prerequisite for relevant research. A wide range of animal models of obesity have been established, including mammals (Mice, Rats, etc.) and non-mammals (e.g., Zebrafish, Nematodes, Drosophila melanogaster, etc.). Obesity is hereditary and there is great heterogeneity among individuals. Multigene models better simulate the pathogenesis of human obesity, and are suitable for comprehensive evaluation of the effectiveness and safety of various treatment strategies. Even under the same genetic background, the individual heterogeneity of the multigene model is still obvious. This individual heterogeneity echoes the different degrees of susceptibility to obesity in different individuals. Protheragen provides a high-quality custom multi-gene mutation model service based on well-established gene editing technology. Our researchers have extensive practical experience in the construction of animal models and offer flexible experimental solutions to ensure that our client's needs are met to the fullest extent.

Tailored Multi-gene Mutation Modeling: a Reliable Tool to Accelerate Obesity Research

Based on the innovative construction strategy and advanced technology of obesity animal models, our custom model service is suitable for gene function analysis, obesity mechanism analysis, drug target discovery, efficacy validation, and other research needs. The specific development process includes the following:

• Customization of Multi-gene Mutation Models

  Various multi-gene models have been developed, such as C57BL/6J mice. We use highly efficient gene editing techniques to edit multiple obesity-related genes, such as knockout, knock-in, etc. Embryonic stem cell-mediated targeting and other gene editing technologies are appropriate means to precisely alter specific sequences in the animal genome. Editable obesity-related genes include appetite and energy regulation-related genes, metabolism-related genes, and so on. We specialize in obesity research and have a wealth of expertise. Rigorous experimental procedures ensure that the constructed models accurately simulate the physiological state after gene mutation.

• Model Validation

  After constructing the multi-gene mutation model, we analyze its physiological and pathological characteristics. In addition, the gene expression profiles, relevant biomarkers, and behaviors of the animal models are also examined to analyze the accuracy of the models. Based on the experimental results and modeling experience, we improve the experimental protocol and further optimize the process of constructing the multi-gene mutation model. The final validated multi-gene mutant model is a reliable tool for evaluating the therapeutic efficacy and safety of anti-obesity drugs and therapies after feeding, physiological indexes measurement, and drug testing.

Workflow

Applications

• Multi-gene mutation models help researchers gain a deeper understanding of the molecular mechanisms of obesity and analyze in detail the role of genes in obesity.
• Multi-gene mutation modeling helps to identify more efficient targets for obesity treatment and provides valuable information for the development of anti-obesity therapies.
• Multigene mutation models are used to study the efficacy and safety of various potential anti-obesity drugs. Physiological and pathological changes in these models are analyzed to understand the potential of drugs to treat obesity and provide guidance for anti-obesity drug optimization.

Advantages

• We provide a one-stop custom multi-gene mutation model service, including protocol design, model construction, incubation to model validation.
• We have rich experience in multiple types of model construction and complete histological, pathological, behavioral, and other testing methods.
• We not only provide customised model services but also provide reliable support for various problems encountered by our clients in obesity research.

Other Quality Services We Provide

We specialize in creating a system of personalized solutions and helping researchers progress smoothly on the path of obesity research. In addition to the construction of multi-gene mutation models, we further provide comprehensive obesity risk assessment, precise diagnostic tests, and obesity prediction services. Detailed analysis of the microbiome of obesity-related biomarkers as well as pathological features plays an indispensable role in obesity research. Our team also provides services based on our extensive testing and analytical experience for a wide range of sample types. As a trusted partner, we also offer highly customized solutions for obesity management and weight control.

Publication Data

Frequently Asked Questions

Which animal lines are used for obesity research?

• C57BL/6J mouse: It is a commonly used multigene model mouse in the laboratory that develops obesity due to overfeeding. Sensitivity to high-fat dietary feeding is usually observed at 6 weeks of age.
• New Zealand obese mouse: This is a polygenic model of obesity and type 2 diabetes. This mouse increases dietary intake and decreases voluntary activity and liver function.

What is the process of constructing a multi-knockout mouse?

• The corresponding gRNA plasmids are constructed against two or more obesity-related target genes. Transcription of the RNAs is followed by prokaryotic microinjection along with Cas mRNA and sequencing is performed to screen for positive F0 generation polygenic heterozygous mice.
• The F0 generation heterozygous mice are mated with wild-type mice to obtain F1 generation two-gene heterozygous mice.
• The two-gene heterozygous mice are selected for crossbreeding to obtain F2 generation mice. The obtained F2 generation mice are identified.

Protheragen provides a custom multi-gene mutation model service by some senior experts, providing strong support for basic obesity research and therapy development. We are at the forefront of meeting our clients' obesity needs, from custom modeling and breeding to phenotyping. Please feel free to contact us to discuss the detailed experimental process if you have any needs in this area.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Chemical-induced Obesity Model
• Custom Chromosome Replacement Model Service
• Custom Single Gene Editing Model Service
• Custom Single Gene Mutation Model Service
• Custom Transgenic Model Service
• Drug Injection-based In Vivo Obesity Model Development Service
• Gene Editing-based In Vivo Obesity Model Development Service
• High Fat-induced Obesity Model
• High Sugar-induced Obesity Model
• Hypothalamic Injury-based In Vivo Obesity Model Development Service
• Mammal Obesity Model
• Non-mammal Obesity Model
• Oophorectomy-based In Vivo Obesity Model Development Service
• Other Nutrient-induced Obesity Model
• Protein-induced Obesity Model