Overview

Obesity is a metabolic disorder caused by an increase in the number of fat cells in the body or an increase in the size of fat cells, leading to an increase in the accumulation of fat in the body. The mechanism of obesity relies heavily on the construction of animal models. Protheragen provides professional In Vivo Models for obesity research. Zebrafish is a kind of non-mammal with a body length of about 3-5 cm. According to the client's needs, our company provides high-quality zebrafish obesity models.

Constructing a Stable Zebrafish Obesity Model with Us

• Modeling

  Our company mainly provides feeding-induced zebrafish obesity models and transgene editing-induced zebrafish obesity models.

  Feeding-induced zebrafish obesity model: Our researchers construct zebrafish obesity models by feeding them a normal diet, high-fat food, high-cholesterol food, and high-protein food. Obesity identification phenotypes differed among the different feeding diets. We provide identification metrics including, but not limited to total cholesterol (TC), triglyceride (TG), fatty liver, hyperlipidemia, fat accumulation, body mass index (BMI), lateral body widening, low-density lipoprotein cholesterol (LDL-C), and weight.

  

  Transgene editing-induced zebrafish obesity model: We use gene editing or microinjection techniques to modify specific genes in zebrafish to study the genetic mechanisms of obesity. Our researchers usually take liver tissues and stain them for observation, measurement of biochemical markers, and obesity gene expression levels.

• Common Indicator Measurement

  The successful construction of the zebrafish obesity model is dependent on the selection of the measurement indexes. Our lab provides the following indexes for testing services:

  • Body weight
  • Glucose
  • Liver observation

  • Length
  • TC
  • Tissue staining

  • BMI
  • TG
  • Computed tomography (CT)-3D scan

Workflow

The process of constructing an obese zebrafish model involves several key steps. First, we select healthy zebrafish with a clear genetic background as experimental subjects. Subsequently, our researchers induce the obese model through feeding or gene editing. During the induction process, the zebrafish are closely observed for weight changes, body size characteristics, and behavioral performance. Afterward, we validate the obesity model by testing biochemical indicators, examining histopathology, and analyzing the expression levels of relevant genes. Finally, based on the collected data and analysis results, our analytical team evaluates the validity and potential application of the model.

Applications

• The zebrafish obesity model can be used to screen zebrafish lipid metabolism-related genes for the discovery of genes that act on poor diets.
• The zebrafish obesity model can be used for the screening of natural anti-obesity drugs.
• The zebrafish obesity model can be used to evaluate drug toxicity and safety.

Advantages of Us

• Combining metabolomics and advanced phenotyping technology, our professional research team helps clients more comprehensively understand the metabolic changes and pathological characteristics of obese zebrafish models.
• According to the different research needs of clients, our experienced gene editing team provides accurate gene manipulation.
• All design plans and data are sent to the client's email address.

Fully Promote Your Anti-Obesity Research

Our anti-obesity research program covers the entire process from the discovery of anti-obesity targets, to the systematic development of advanced therapies, to the rigorous evaluation of preclinical studies. In this process, we rely on an elite team of senior scientists and industry experts who are committed to providing clients with customized, efficient and scientific solutions based on their outstanding professionalism and rigorous scientific attitudes.

Learn More about Obesity Services

Our company focuses on the development of customized anti-obesity therapies for different needs worldwide. With the help of cutting-edge bioanalytical technology and a large database, our expert team has explored gene expression, diagnostic tests, biomarker identification, etc., related to obesity and other metabolic diseases, to realize the precise physiological or pathological assessment of obesity. In addition, we have revealed the underlying mechanisms of obesity through microbial community analysis and pathophysiologic studies.

Publication Data

Frequently Asked Questions

Why choose zebrafish as an animal model?

As an experimental animal, zebrafish has many significant advantages. First of all, its small size makes it easy to conduct fine observations under a microscope, which provides great convenience for high-precision research such as micropore experiments. Secondly, zebrafish reproduce through in vitro fertilization, and the embryos are highly transparent, making it easy to observe the developmental morphology of zebrafish embryos. What is particularly important is that the genetic sequence of zebrafish is highly similar to that of humans, reaching a similarity of 87%. In addition, zebrafish also have strong reproductive capabilities.

How do lipid-lowering substances in zebrafish affect lipid metabolism?

Lipid-lowering substances in zebrafish effectively affect the lipid metabolism process by inducing the autophagy system and regulating cholesterol metabolism and synthesis pathways.

As a professional anti-obesity therapy research company, Protheragen provides high-quality zebrafish obesity models for preclinical research. We are committed to solving all problems encountered by clients in modeling. Please feel free to contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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