Overview

Obesity is a metabolic disorder caused by a combination of genetic and environmental factors. Animal Models are indispensable research objects to further understand the pathogenesis of obesity and explore effective treatment methods. At present, the construction of animal obesity models mainly includes high-fat diet-induced obesity models, drug- or surgery-induced obesity models, spontaneous or genetic obesity models, transgenic animal models, and so on. Transgenic animal models (Mouse, Rat, Zebrafish, etc.) play a crucial role in the functioning of key genes. Protheragen is actively involved in new hotspots and technologies related to obesity and provides a one-stop custom transgenic model service. We are skilled in a wide range of animal experimentation techniques and are committed to providing reliable support in obesity-related research.

Unlocking Obesity Insights Through Tailored Transgenic Modeling Solutions

The advantage of the transgenic obesity model is to observe the biological effects of gene overexpression or knockout in animals. We have a professional team with rich experience in the construction of animal models of obesity, according to the specific needs of the research to develop appropriate transgenic model construction schemes. The construction process includes the following:

• Customization of Transgenic Models

  We inject exogenous genes into fertilized eggs or stem cells of animal models by microinjection and implant them into recipient animals to develop them into animals carrying the exogenous genes and capable of stable inheritance. By analyzing the correlation between the transgene and the phenotype, the function of the exogenous gene is analyzed, and studies on the molecular mechanism of obesity and anti-obesity therapies are conducted.

  • Overexpression transgene model: We construct an expression vector with the target gene and promoter. Then the vector is injected into the fertilized eggs of animals using microinjection. We achieve broad and specific expression of the transgene by constructing different promoters, such as broad and tissue-specific promoters.
  • RNA interference (RNAi) transgene model: Short hairpin RNA (shRNA) vectors are constructed and injected into fertilized eggs by microinjection. The interference effect of shRNAi is used to silence the expression of target genes.
  • Bacterial artificial chromosome (BAC) transgenic model: BACs are effective tools for processing large segments of DNA and better explain the important functions of transgenes. BACs are either injected directly into prokaryotic microinjections or modified and injected into fertilized eggs in animal models to obtain transgenic animals. The model is used to study gene structure, spatiotemporal-specific gene expression, regulatory elements, etc.
  • Other types of transgenic models

• Validation of the Model

  Based on the perfect testing technology, the constructed transgenic model is tested for physiology, pathology, gene expression, and so on. The accuracy of the transgenic models is assessed by all-round analysis. These transgenic models are reliable tools for studying the molecular mechanism of obesity and evaluating therapies.

Workflow

Applications

• Transgenic models simulate the process of obesity more accurately and are used to study in depth the role of genes in obesity and the mechanisms of obesity.
• Transgenic modeling plays a crucial role in evaluating the safety and efficacy of anti-obesity therapies targeting specific targets and helps accelerate the therapy development process.
• Transgenic modeling is used to identify more effective therapeutic targets and as a basis for the development of anti-obesity drugs.

Advantages

• We conduct rational model design and detailed project assessment after understanding the client's needs. Specific project history and timeline will be provided at the time of communication.
• We have a strict quality control system to make transgenic model construction more efficient.
• We have experienced scientists in charge of transgenic model construction. In addition to timely communication of project progress, we also provide uninterrupted research support.

Other Cutting-edge Services We Provide

Publication Data

Frequently Asked Questions

What are the common techniques used for the construction of transgenic mouse models?

• DNA prokaryotic microinjection method: Exogenous genes are directly injected into fertilized eggs using microinjection so that the exogenous genes are integrated into the DNA of the recipients and develop into transgenic animals. This method is more widely used.
• Embryonic stem cell blastocyst microinjection: The exogenous gene is introduced into embryonic stem cells in vitro, and the transgenic embryonic stem cells are then injected into the blastocyst of the animal. This embryonic stem cell participates in the embryo formation of the host, forming chimeras until germline chimerism is achieved. Currently, the embryonic stem cell-mediated method is well-established in mice. Knockout and capture of genes are commonly used in this type of method.

What are the considerations when constructing transgenic animals?

Generally, the region of transgene expression depends on the promoter. Using a systemically expressed promoter will result in a systemically expressed transgenic animal. If a promoter for tissue-specific expression of the gene is chosen, tissue-specific expression of the transgenic mouse will be obtained. Due to the randomness of insertion of the transgene fragments, it is possible to insert into some repressive regions so that the gene is not expressed, or to insert into some enhancement regions so that the gene is highly expressed, and multicopy insertion usually occurs.

Protheragen provides a custom transgenic model service to meet different experimental needs with mature technology and a perfect experimental process. Our rigorous and professional technical team will develop customized solutions and communicate with you about the progress of your project. Please feel free to contact us to learn more about the construction process and explore obesity with us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Chemical-induced Obesity Model
• Custom Chromosome Replacement Model Service
• Custom Multi-Gene Mutation Model Service
• Custom Single Gene Editing Model Service
• Custom Single Gene Mutation Model Service
• Drug Injection-based In Vivo Obesity Model Development Service
• Gene Editing-based In Vivo Obesity Model Development Service
• High Fat-induced Obesity Model
• High Sugar-induced Obesity Model
• Hypothalamic Injury-based In Vivo Obesity Model Development Service
• Mammal Obesity Model
• Non-mammal Obesity Model
• Oophorectomy-based In Vivo Obesity Model Development Service
• Other Nutrient-induced Obesity Model
• Protein-induced Obesity Model