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Ferric reducing antioxidant power (FRAP) assay kit
Cat. No.:
OB0125WXX-056
Assay Type:
Antioxidant assay
Target Species:
Universal
Assay Target:
FRAP
Size:
Product Overview
Description:
Ferric reducing antioxidant power (FRAP) assay kit is an antioxidant capacity assay kit that uses ferrous standards to determine the antioxidant content of a sample. Each kit provides sufficient reagents to perform up to 200 assays, providing a simple and efficient analytical method for assessing antioxidant status in response to disease, diet, or other physiological changes.
Assay Principle:
In the assay, Fe3+ is initially reduced to the ferrous form (Fe2+) by an electron-donor antioxidant in the sample. The reduction of the Fe colorimetric probe complex produces a dark blue product that can be measured at 540-600 nm. Samples can be compared to iron standards to determine antioxidant potential.
Background:
Oxidative stress is a physiological state in which there is an imbalance between reactive oxygen species (ROS) and antioxidant concentrations. Under normal physiological conditions, cellular production of ROS can be counteracted by the action of cellular antioxidant enzymes, macromolecules or micromolecules, and other redox molecules. Excessive ROS accumulation leads to inflammation and cellular damage and has been implicated in the development of a variety of diseases, and it is essential to neutralize and remove excess ROS from cells in a timely manner through a variety of antioxidant defense mechanisms. Antioxidants are molecules that contain one or more free electrons that can be donated to stabilize ROS. They may be present transiently in different tissues or cells. It is essential to test for antioxidant capacity.
Synonyms:
FRAP assay
Applications:
Ferric reducing antioxidant power (FRAP) assay kit is a quantitative assay for the measurement of antioxidant potential in a wide variety of samples such as serum, plasma, saliva, tissue homogenates, urine, tears, cell extracts, and purified food or drug extracts.
Research Area:
Antioxidant research; Obesity
Specification
Sample Type:
Serum; Plasma; Urine; Saliva; Tears; Tissue homogenates; Cell extracts; Purified food
Kit Components:
Colorimetric probe; Iron chloride solution; Iron (II) standard; Assay buffer (5X)
Detection Method:
Colorimetric method
Detection Limit:
4 µM Fe2+ iron equivalents
Storage:
Store at 4°C.
