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Vitamin B5 (Pantothenic acid) test kit
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Cat. No.:
OB0125WXX-022
Synonyms:
VB5 test kit; Pantothenic acid test kit
Size:
96T
Specification
Description:
Vitamin B5 (Pantothenic acid) test kit is a nutritional test kit that uses the microbiology method to detect the volume of VB5 in food matrices.
Background:
VB5, one of the essential vitamins, is found in grains, meat, legumes, vegetables, eggs, and milk. It is involved in the synthesis of coenzyme A (CoA), which is essential for cellular energy production and the synthesis and degradation of proteins, carbohydrates, and fats.
Assay Type:
Quantitative assay
Assay Principle:
VB5 supplies the growth of Lactiplantibacillus plantarum. Its growth rate is associated with the volume of VB5 within the sample. The VB5 medium (Lactiplantibacillus plantarum added), samples, or standards are taken and added to the microwell. Afterward, they are incubated at 37°C and protected from light. Lactiplantibacillus plantarum will start growing until VB5 is consumed entirely. It takes 24 hours to complete incubation. Then, a microplate reader is used to read the sample turbidity with a 610-630 nm or 540-550 nm filter. The volume of VB5 in the sample is determined by comparing the sample turbidity with the standards.
Assay Target:
VB5
Research Area:
Food safety; VB5 research; Obesity
Sample Type:
Fruit juices; Drinks; Jelly; Milk powder; Baby food; Bakery food; Wheat flour; Dairy product; Cereal
Kit Components:
Microwell plate; Cover film; Plate rack; Reagent reservoir; Package insert; Testing report; Sterile water; Pantothenic acid medium; Calcium pantothenate standards; Pantothenic acid assay strain
Applications:
Vitamin B5 (Pantothenic acid) test kit is used for quantitative analysis of vitamin B5 (pantothenic acid) in food matrices (added and native) to assess the impact of nutritional status on body weight.
Storage:
Store at 2-8°C.