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Uric acid assay kit (Colorimetric method - 250T)
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Cat. No.:
OB0125WXX-020
Synonyms:
Uric acid assay kit; Uric acid
Size:
250T
Specification
Description:
Uric acid assay kit is a metabolic assay kit for the detection of uric acid with a linear detection range of 0.22 mg/dL (13 ) to 30 mg/dL (1785), and is sensitive, accurate, and can be performed directly on serum samples.
Background:
Uric acid is a purine metabolite that is degraded to allantoin by uricase in most mammals. It is the end product of purine metabolism in humans due to the lack of the uric acid oxidase gene, and therefore, human blood levels of uric acid are higher than in most mammals. Uric acid is an important physiological plasma antioxidant that effectively protects biological targets from oxidation by hydroxyl radicals, hypochlorous acid, and peroxynitrite.
Assay Type:
Quantitative assay
Assay Principle:
This uric acid test utilizes 2,4,6-tripyridine-s-triazine to form a blue complex with iron in the presence of uric acid. The uric acid concentration in serum is calculated from the intensity of the color measured at 590 nm. This method allows direct detection of uric acid in serum without any pre-treatment. The optimized formulation greatly reduces the interference of substances in the original sample.
Assay Target:
Uric acid
Target Species:
Universal
Research Area:
Kidney biomarkers; Oxidative stress; ATP and related research; Obesity
Sample Type:
Serum; Plasma; Urine; Other biological samples
Kit Components:
Reagent A; Reagent B; Reagent C; Standard; 10 mg/dL uric acid blank control
Detection Limit:
0.2 mg/dL (13 )
Reaction Time:
30 min
Applications:
Uric acid assay kit is used for the quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.
Storage:
Store at 4°C (reagents) and -20°C (standard and blank control).