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Total antioxidant capacity (TAC) assay kit
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Cat. No.:
OB0125WXX-060
Synonyms:
TAC assay kit; Total antioxidant capacity (TAC)
Size:
200T
Specification
Description:
Total antioxidant capacity (TAC) assay kit is an antioxidant capacity assay kit that measures the total antioxidant capacity of biomolecules in a variety of samples by the SET mechanism, making it an ideal tool for efficiently and accurately assessing reducing or antioxidant capacity.
Background:
Oxidative stress is a physiological state of imbalance between reactive oxygen species (ROS) and antioxidant concentrations. Excess ROS accumulation can lead to DNA, protein, and cellular damage and is associated with a variety of diseases. These excess ROS must be excreted from the body promptly through various antioxidant defense mechanisms. It is important to assess the antioxidant capacity of biological fluids, cells, and extracts. Antioxidants typically neutralize free radicals through hydrogen atom transfer (HAT) or single electron transfer (SET) mechanisms. The single electron transfer assay quantifies the ability of an antioxidant to transfer one electron to reduce any compound (e.g., free radicals, carbonyls, and metals).
Assay Principle:
In a 96-well microplate, the sample is compared to a uric acid standard of known concentration. The samples and standards are diluted with the reaction reagent, and the reaction is carried out for a few minutes after the addition of copper. After the reaction is stopped, a standard 96-well spectrophotometric microplate reader is used to take a reading at 490 nm. Antioxidant capacity is determined by comparison with a uric acid standard.
Assay Target:
TAC
Target Species:
Universal
Research Area:
Antioxidant research; Obesity
Sample Type:
Cell lysate; Plasma; Serum; Urine; Tissue homogenates; Food extracts
Kit Components:
Uric acid standard; Reaction buffer (100X); Copper ion reagent (100X); Stop solution
Applications:
Total antioxidant capacity (TAC) assay kit is fast and reliable for the direct determination of total antioxidant capacity in cell lysates, plasma, tissue homogenates, serum, urine, and food extracts.
Storage:
Store at 4°C (reaction buffer) and room temperature (others).