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Thiobarbituric acid reactive substances (TBARS) assay kit (Fluorometric, 2.6-100 μmol/L)
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Cat. No.:
OB0525WXX-402
Synonyms:
Thiobarbituric acid reactive substances; TBARS assay kit; Thiobarbituric acid reactive substances (TBARS); TBARS
Size:
96T
Specification
Description:
Thiobarbituric acid reactive substances (TBARS) assay kit (Fluorometric, 2.6-100 μmol/L) is an assay kit for the rapid and sensitive detection of TBARS in the range of 2.6-100 μmol/L.
Background:
The organism generates oxygen free radicals through enzymatic and non-enzymatic systems, which attack polyunsaturated fatty acids in biological membranes and induce lipid peroxidation, resulting in the formation of lipid peroxides. Malondialdehyde (MDA) is one of the common products of lipid peroxidation in organisms. It is a biomarker of lipid peroxidation, which can reflect the degree of lipid peroxidation in organisms and indirectly the degree of cellular damage.
Assay Principle:
Succinic acid is converted to pyruvic acid, which reacts with specific reagents and dyes to produce a colored product. The color intensity of the reaction product at 570 nm or fluorescence at Ex/Em = 530/585 nm is proportional to the concentration of succinic acid in the sample.
Assay Target:
TBARS
Target Species:
Universal
Research Area:
Oxidative stress; Lipid peroxidation; Obesity research
Sample Type:
Serum; Plasma; Animal tissue
Kit Components:
Clarificant; Acid reagent; TBA reagent; Standard solution (200 μmol/L); Microplate; Plate sealer
Detection Range:
2.6-100 μmol/L
Sensitivity:
0.85 μmol/L
Precision (Intra-assay):
0.071
Precision (Inter-assay):
0.043
Reaction Time:
80 min
Recovery:
1.009
Stability:
6 Months
Applications:
Thiobarbituric acid reactive substances (TBARS) assay kit (Fluorometric, 2.6-100 μmol/L) is used to measure TBARS concentration in serum, plasma, animal tissue, culture cells, and other samples.
Storage:
Store at 2-8°C.