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Succinate assay kit
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Cat. No.:
OB0525WXX-403
Synonyms:
Succinate test kit; Succinate
Size:
100T
Specification
Description:
Succinate assay kit is a metabolic assay kit for the rapid and sensitive detection of succinate.
Background:
Succinic acid is found in all animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy production. Due to its low toxicity, succinic acid is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries.
Assay Type:
Quantitative assay
Assay Principle:
Tetrazolium salt MTT is reduced to reduced MTT in the NADH-coupled enzymatic reaction and shows an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Assay Target:
Succinate
Target Species:
Universal
Research Area:
TCA cycle; Obesity research; Food safety and analysis
Sample Type:
Cells; Tissue
Kit Components:
Assay buffer; Enzyme Mix; Co substrate; PEP; Standard
Detection Limit:
10 µM (OD); 2 µM (FL)
Reaction Time:
30 min
Stability:
6 Months
Applications:
Succinate assay kit is used to measure succinate (succinic acid) in food, beverages, agricultural products, and other biological samples.
Storage:
Store at 2-8°C.