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Reduced glutathione (GSH) assay kit (Colorimetric)
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Cat. No.:
OB0525WXX-405
Synonyms:
Reduced glutathione; GSH assay kit; Reduced glutathione (GSH); GSH
Size:
96T
Specification
Description:
Reduced glutathione (GSH) assay kit (Colorimetric) is an assay kit for the rapid and sensitive detection of GSH in the range of 2-100 μmol/L.
Background:
Reduced glutathione (GSH) is a low molecular scavenger of O2-, H2O2, and LOOH.GSH is a small molecular peptide composed of glutamate, glycine, and cysteine, and is the major nonprotein thiol compound in tissues. GSH is a substrate for GSH-Px and GSH-ST, which are indispensable for the decomposition of hydrogen peroxide by these enzymes and stabilize thiol-containing enzymes. GSH plays an important role in the prevention, rehabilitation, and therapeutic research of diseases. When GSH is deficient or depleted, it may lead to the production of toxic effects or increase the toxic effects of many chemicals or environmental factors. This may be associated with an increase in oxidative damage, making GSH levels an important factor in measuring the body's antioxidant capacity.
Assay Principle:
Reduced glutathione (GSH) reacts with dinitrobenzoic acid (DNTB) to form a yellow complex, which can be detected at 405 nm, and the amount of reduced glutathione calculated indirectly.
Assay Target:
GSH
Target Species:
Universal
Research Area:
Oxidative stress; Antioxidant; Obesity research
Sample Type:
Serum; Plasma; Cell culture supernatant; Tissue; Cells
Kit Components:
Acid reagent; Phosphate; DTNB solution; GSH standard; GSH standard stock diluent; Microplate; Plate sealer
Detection Range:
2-100 μmol/L
Sensitivity:
2 μmol/L
Precision (Intra-assay):
0.032
Precision (Inter-assay):
0.019
Reaction Time:
25 min
Recovery:
0.96
Stability:
6 Months
Applications:
Reduced glutathione (GSH) assay kit (Colorimetric) is used to measure GSH content in serum, plasma, cells, cell culture supernatant, and tissue samples.
Storage:
Store at 2-8°C.