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Rat beta-3 adrenergic receptor (ADRB3) ELISA kit (0.156-10 ng/mL, 0.062 ng/mL)
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Cat. No.:
OB0525WXX-346
Synonyms:
Rat ADRB3 ELISA kit; Rat beta-3 adrenergic receptor (ADRB3); Rat beta-3 adrenergic receptor; Beta-3 adrenergic receptor (ADRB3); Rat ADRB3; ADRB3 ELISA kit
Size:
48T; 96T
Specification
Description:
Rat beta-3 adrenergic receptor (ADRB3) ELISA kit (0.156-10 ng/mL, 0.062 ng/mL) is a ready-to-use ELISA kit for analyzing rat ADRB3 levels with a detection range of 0.156-10 ng/mL and a sensitivity of 0.062 ng/mL.
Background:
ADRB3, the β3-adrenergic receptor, regulates lipolysis, thermogenesis in brown adipose tissue, and vasodilation. β3-adrenergic receptor agonists have been found to have significant anti-obesity and anti-diabetic effects. ADRB3 is an attractive target in anti-obesity research.
Formula Weight:
43,146 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with an antibody specific for ADRB3. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADRB3. Avidin conjugated to HRP is then added to each microplate well and incubated. Only wells containing ADRB3, biotin-conjugated antibody and enzyme-conjugated avidin will show a color change after addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADRB3 in the sample is then determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Sandwich
Assay Target:
ADRB3
Target Species:
Rat
Research Area:
Thermogenesis; Lipolysis; Signaling pathways; Metabolism; Adipocyte differentiation; Signal transduction; Obesity
Sample Type:
Tissue homogenates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.062 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Rat beta-3 adrenergic receptor (ADRB3) ELISA kit (0.156-10 ng/mL, 0.062 ng/mL) is designed for the in vitro quantitative analysis of ADRB3 levels in rat tissue homogenates, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).