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Rat adrenocorticotropic hormone (ACTH) CLIA kit
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Cat. No.:
OB0125WXX-028
Synonyms:
ACTH CLIA kit; Rat ACTH; Rat adrenocorticotropic hormone (ACTH); Adrenocorticotropic hormone (ACTH)
Size:
24T; 48T; 96T
Specification
Description:
Rat adrenocorticotropic hormone (ACTH) CLIA kit is a CLIA kit assay for the detection of rat ACTH levels. The range of the assay is 11.72-3000 pg/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Type:
Competitive inhibition
Assay Principle:
The microtiter plates in this kit are pre-coated with a monoclonal antibody specific for ACTH. A competitive inhibitory reaction occurs between biotin-labeled ACTH and unlabeled ACTH (standards or samples) and the pre-coated ACTH-specific antibody. After incubation, the unbound conjugate is washed away. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. The amount of bound HRP is inversely proportional to the concentration of ACTH in the sample. A mixture of substrates A and B is then added to produce glow emission kinetics. After development, the intensity of the glow is inversely proportional to the amount of ACTH in the sample or standard.
Assay Target:
ACTH
Target Species:
Rat
Research Area:
Hormone research; Circadian rhythm; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
11.72-3000 pg/mL
Sensitivity:
4.52 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Rat adrenocorticotropic hormone (ACTH) CLIA kit is used to detect rat ACTH levels in serum, plasma, and other biological fluid samples by chemiluminescent immunoassay.
Storage:
Store at 2-8°C.