Products
Online Inquiry
Rat adiponectin (ADP) ELISA kit (<10 pg/mL)
Inquiry
Cat. No.:
OB0525WXX-227
Synonyms:
Rat ADP ELISA kit; Rat adiponectin (ADP); Rat adiponectin; Adiponectin (ADP); Rat ADP; ADP ELISA kit
Size:
96T
Specification
Description:
Rat adiponectin (ADP) ELISA kit (<10 pg/mL) is a ready-to-use ELISA kit for analyzing rat ADP levels with a sensitivity of <10 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,410 Da
Assay Principle:
The assay utilizes an antibody specific for rat ADP coated on a 96-well plate. Standards and samples are pipetted into the wells, and the ADP in the samples is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-rat ADP antibody is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, TMB substrate solution is added to the wells, and the color is then developed proportionally to the amount of bound ADP. The termination solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
ADP
Target Species:
Rat
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Cell culture supernatants; Serum; Plasma; Tissue homogenates; Urine
Sensitivity:
<10 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
12 Months
Applications:
Rat adiponectin (ADP) ELISA kit (<10 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in rat cell culture supernatants, serum, plasma, tissue homogenates, and urine samples.
Storage:
Store at 2-8°C.