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Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.78-50 ng/mL, 0.31 ng/mL)
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Cat. No.:
OB0525WXX-112
Synonyms:
Rat ACP5 ELISA kit; Rat acid phosphatase 5, tartrate resistant (ACP5); Rat acid phosphatase 5, tartrate resistant; ACP5 ELISA kit; Rat ACP5; Phosphatase 5, tartrate resistant (ACP5); EC 3.1.3.2
Size:
24T; 48T; 96T
Specification
Description:
Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.78-50 ng/mL, 0.31 ng/mL) is a ready-to-use ELISA kit for analyzing rat ACP5 levels with a detection range of 0.78-50 ng/mL and a sensitivity of 0.31 ng/mL.
Background:
ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.
Formula Weight:
36,599 Da
Assay Principle:
The detection principle used in this kit is a sandwich enzyme immunoassay. The microtiter plate provided in the kit is pre-coated with an antibody specific for ACP5. Standards or samples are added to the wells of the microtiter plate together with a biotin-conjugated antibody specific for ACP5. Avidin conjugated to HRP is then added to each microplate well and incubated. Upon addition of the TMB substrate solution, only wells containing ACP5, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ACP5 in the sample can then be determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Double antibody sandwich
Assay Target:
ACP5
Target Species:
Rat
Research Area:
Hormone regulation; Metabolites; Defense response; Inflammatory; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Other biological fluids
Detection Range:
0.78-50 ng/mL
Sensitivity:
0.31 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.78-50 ng/mL, 0.31 ng/mL) is designed for the in vitro quantitative analysis of ACP5 levels in rat serum, plasma, tissue homogenates, and other biological fluid samples.
Storage:
Store at 2-8°C.