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Pyruvate assay kit (Colorimetric or fluorometric)
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Cat. No.:
OB0525WXX-408
Synonyms:
Pyruvate assay kit; Pyruvate
Size:
100T
Specification
Description:
Pyruvate assay kit (Colorimetric or fluorometric) is a metabolic assay kit for the rapid and sensitive detection of pyruvate.
Background:
Pyruvate is a key intermediate in the cellular metabolic pathway. It can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy via acetyl-CoA, and to the amino acids alanine and ethanol. Abnormal pyruvate levels are associated with liver disease and metabolic disorders. Simple, straightforward, and automated procedures for measuring pyruvate concentration have a wide range of applications in research and drug discovery.
Assay Type:
Quantitative assay
Assay Principle:
Pyruvate oxidase and hydrogen peroxide can be measured in one step using a single working reagent. The color intensity of the reaction product at 570nm or the fluorescence intensity at Ex/Em = 530/585nm is proportional to the pyruvate concentration in the sample.
Assay Target:
Pyruvate
Target Species:
Universal
Research Area:
Glycolysis and carbohydrates; TCA cycle; Obesity research; Food safety and analysis; Amino acid and protein
Sample Type:
Biological samples
Kit Components:
Enzyme Mix; Dye reagent; Standard; Pyruvate
Detection Limit:
2 μM (OD); 0.2 μM (FL)
Reaction Time:
20 min
Stability:
6 Months
Applications:
Pyruvate assay kit (Colorimetric or fluorometric) is used to detect pyruvate in biological samples, and evaluate of drugs on its metabolism.
Storage:
Store at 2-8°C.