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Porcine adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL)
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Cat. No.:
OB0525WXX-186
Synonyms:
Porcine ACTH ELISA kit; Porcine adrenocorticotropic hormone (ACTH); Porcine adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Porcine ACTH
Size:
48T; 96T
Specification
Description:
Porcine adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL) is a ready-to-use ELISA kit for analyzing porcine ACTH levels with a detection range of 15.6-1000 pg/mL and a sensitivity of 5 pg/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
The double sandwich ELISA technique is used in this experiment, and the ELISA kit provided is a typical double sandwich ELISA kit. The pre-coated antibody is a monoclonal antibody against porcine ACTH, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue under the catalysis of peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
ELISA Type:
Sandwich
Assay Target:
ACTH
Target Species:
Porcine
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Porcine serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant ACTH concentration
Detection Range:
15.6-1000 pg/mL
Sensitivity:
5 pg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Porcine adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in porcine serum, plasma, cell culture supernatant, organizations in the natural and recombinant ACTH concentration samples.
Storage:
Store at 2-8°C.