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Porcine adiponectin receptor 1 (ADIPOR1) ELISA kit
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Cat. No.:
OB0525WXX-239
Synonyms:
Porcine ADIPOR1 ELISA kit; Porcine adiponectin receptor 1 (ADIPOR1); Porcine adiponectin receptor 1; Adiponectin receptor 1 (ADIPOR1); Porcine ADIPOR1; ADIPOR1 ELISA kit
Size:
48T; 96T
Specification
Description:
Porcine adiponectin receptor 1 (ADIPOR1) ELISA kit is a ready-to-use ELISA kit for analyzing porcine ADIPOR1 levels with a detection range of 1.0-25 ng/mL and a sensitivity of 0.1 ng/mL.
Background:
ADIPOR1 is a receptor for adiponectin, a hormone secreted by adipocytes that regulates fatty acid catabolism and blood glucose levels. It has been shown to activate the AMPK signaling pathway, which plays an important role in the regulation of glucose and lipid metabolism as well as inflammation and oxidative stress in vivo. ADIPOR1 is an attractive target for anti-obesity research.
Formula Weight:
42,616 Da
Assay Principle:
The kit is based on a competitive enzyme immunoassay technique using an anti-ADIPOR1 monoclonal antibody and an ADIPOR1-HRP conjugate. The assay sample and buffer are incubated with the ADIPOR1-HRP Conjugate on a pre-coated plate for one hour. At the end of the incubation period, the samples are decanted, and the wells are washed 5 times. The wells are then incubated with HRP enzyme substrate. The products of the enzyme-substrate reaction form a blue color complex. Finally, the reaction is stopped by the addition of a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of ADIPOR1 because ADIPOR1 in the sample competes with the ADIPOR1-HRP conjugate for the binding site of the anti-ADIPOR1 antibody. Since the number of binding sites is limited, as more sites are occupied by ADIPOR1 in the sample, fewer sites remain to bind to the ADIPOR1-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of ADIPOR1 in each sample is derived from this standard curve.
Assay Target:
ADIPOR1
Target Species:
Porcine
Research Area:
Lipid metabolism; Signaling pathways; Obesity
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range:
1.0-25 ng/mL
Sensitivity:
0.1 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Porcine adiponectin receptor 1 (ADIPOR1) ELISA kit is designed for the in vitro quantitative analysis of ADIPOR1 levels in porcine serum, plasma, cell culture supernatants, body fluid, and tissue homogenate samples.
Storage:
Store at 2-8°C.