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Porcine adiponectin (ADP) ELISA kit (6.25-400 ng/mL)
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Cat. No.:
OB0525WXX-270
Synonyms:
Porcine ADP ELISA kit; Porcine adiponectin (ADP); Porcine adiponectin; Adiponectin (ADP); Porcine ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Porcine adiponectin (ADP) ELISA kit (6.25-400 ng/mL) is a ready-to-use ELISA kit for analyzing porcine ADP levels with a detection range of 6.25-400 ng/mL. Its sensitivity is less than 2.51 ng/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,370 Da
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADP. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Quantitative sandwich
Assay Target:
ADP
Target Species:
Porcine
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
6.25-400 ng/mL
Sensitivity:
<2.51 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Porcine adiponectin (ADP) ELISA kit (6.25-400 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in porcine serum, plasma, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).