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Pig adiponectin (ADP) ELISA kit (1.875-30 μg/mL)
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Cat. No.:
OB0525WXX-268
Synonyms:
Pig ADP ELISA kit; Pig adiponectin (ADP); Pig adiponectin; Adiponectin (ADP); Pig ADP; ADP ELISA kit
Size:
24T; 48T; 96T
Specification
Description:
Pig adiponectin (ADP) ELISA kit (1.875-30 μg/mL) is a ready-to-use ELISA kit for analyzing pig ADP levels with a detection range of 1.875-30 μg/mL. Its sensitivity is less than 1.875 μg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The assay uses a competitive inhibition enzyme immunoassay technique. The microtiter plate provided in the kit is pre-coated with an antibody specific for ADP. Standards or samples are added to the wells of the microtiter plate along with biotin-conjugated ADP, and a competitive inhibition reaction occurs between the ADP (standard or sample) and biotin-conjugated ADP and the pre-coated ADP-specific antibody. The higher the amount of ADP in the sample, the less biotin-conjugated ADP will bind to the antibody. After washing, avidin-conjugated HRP is added to the wells. A substrate solution is added to the wells, and the color develops inversely to the amount of ADP in the sample. The color development is stopped, and the color intensity is measured.
ELISA Type:
Quantitative competitive
Assay Target:
ADP
Target Species:
Pig
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates
Detection Range:
1.875-30 μg/mL
Sensitivity:
<1.875 μg/mL
Precision (Intra-assay):
CV<15%
Precision (Inter-assay):
CV<15%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Pig adiponectin (ADP) ELISA kit (1.875-30 μg/mL) is designed for the in vitro quantitative analysis of ADP levels in pig serum, plasma, and tissue homogenate samples.
Storage:
Store at 2-8°C.