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Oxidized glutathione (GSSG) assay kit (Colorimetric)
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Cat. No.:
OB0525WXX-396
Synonyms:
GSSG assay kit; Oxidized glutathione (GSSG); Oxidized glutathione; GSSG
Size:
96T
Specification
Description:
Oxidized glutathione (GSSG) assay kit (Colorimetric) is a metabolic assay kit for the rapid and sensitive detection of GSSG in the range of 0.36-30 μmol/L.
Background:
Glutathione is a tripeptide and the most common intracellular thiol. In the cell, glutathione exists in two forms: reduced (GSH) and oxidized (GSSG). Under physiological conditions, more than 98% of intracellular glutathione is GSH because GSSG is rapidly reduced to GSH by glutathione reductase. The GSH-GSSG system is the most abundant redox system in eukaryotic cells. It plays an important role in cellular homeostasis and is involved in apoptosis-related signaling.
Assay Type:
Quantitative assay
Assay Principle:
GSSG is reduced to GSH by glutathione reductase, and GSH reacts with DTNB to produce GSSG and yellow TNB. Total glutathione (GSSG+GSH) determines the amount of yellow TNB. Therefore, total glutathione can be calculated by measuring the OD value at 412 nm. Care should be taken to remove GSH from the sample using appropriate reagents and then determine the GSSG content according to the reaction principle described above.
Assay Target:
GSSG
Target Species:
Universal
Research Area:
Oxidative stress; Antioxidant; Obesity research
Sample Type:
Serum; Plasma; Animal tissue; Red blood cells; Cultured cells
Kit Components:
Buffer solution; Standard; Protein precipitator; Enzyme stock solution; Chromogenic agent; Diluent; GSH scavenger auxiliary solution; GSH scavenger; Substrate; Microplate; Plate sealer
Detection Range:
0.36-30 μmol/L
Sensitivity:
0.36 μmol/L T-GSH
Precision (Intra-assay):
0.039
Precision (Inter-assay):
0.006
Reaction Time:
100 min
Recovery:
0.97
Stability:
6 Months
Applications:
Oxidized glutathione (GSSG) assay kit (Colorimetric) is used to measure T-GSH and GSSG content in serum (plasma), animal tissue, red blood cells, and cultured cell samples.
Storage:
Store at 2-8°C.