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Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L)
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Cat. No.:
OB0525WXX-412
Synonyms:
Non-esterified free fatty acids; NEFAs assay kit; Non-esterified free fatty acids (NEFAs); NEFAs
Size:
96T
Specification
Description:
Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L) is a metabolic assay kit for the rapid and sensitive detection of NEFA in the range of 0.15-1.5 mmol/L.
Background:
Free fatty acids, also known as non-esterified fatty acids (NEFA), are derived from the diet or metabolism of adipose tissue. In adipose tissue, hormone-sensitive lipase (HSL) breaks down triglycerides to produce glycerol and fatty acids. When circulating in the body, free fatty acids are bound to plasma albumin and serve as a source of energy that is readily absorbed by muscle, brain, and other tissues and organs. Non-esterified free fatty acid (NEFA) is not only a product of fat hydrolysis, but also a substrate for fat synthesis. The concentration of NEFA has been linked to lipid metabolism, glucose metabolism, and endocrine function.
Assay Type:
Quantitative assay
Assay Principle:
Under weak acidic conditions, NEFA reacts with nantokite to form copper soap, which has a specific absorption peak at 715 nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
Assay Target:
NEFA
Target Species:
Universal
Research Area:
Obesity research
Sample Type:
Tissue; Cells
Kit Components:
Extracting solution; Palmitic acid standard (10 mmol/L); Control solution; Reaction solution; Microplate; Plate sealer
Detection Range:
0.15-1.5 mmol/L
Sensitivity:
0.15 mmol/L
Precision (Intra-assay):
0.051
Precision (Inter-assay):
0.033
Reaction Time:
30 min
Recovery:
1.01
Stability:
6 Months
Applications:
Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L) is used to measure the NEFAs content in animal tissue samples.
Storage:
Store at 2-8°C.