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Mouse medium-chain specific acyl-CoA dehydrogenase (ACADM) ELISA kit (0.312-20 ng/mL)
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Cat. No.:
OB0525WXX-064
Synonyms:
Mouse ACADM ELISA kit; Mouse medium-chain specific acyl-CoA dehydrogenase; Medium-chain specific acyl-CoA dehydrogenase (ACADM); ACADM ELISA kit; Mouse ACADM; EC 1.3.8.7
Size:
48T; 96T
Specification
Description:
Mouse medium-chain specific acyl-CoA dehydrogenase (ACADM) ELISA kit (0.312-20 ng/mL) is a ready-to-use ELISA kit for analyzing mouse ACADM levels with a detection range of 0.312-20 ng/mL and a sensitivity of 0.156 ng/mL.
Background:
ACADM is an enzyme that catalyzes the first step of mitochondrial fatty acid oxidation. It may serve as a target to regulate fatty acid metabolism for the treatment of obesity, cancer, and other related diseases.
Formula Weight:
46,481 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with an antibody specific for ACADM. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated ACADM-specific polyclonal antibody preparation, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing ACADM, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ACADM in the sample is then determined by comparing the OD of the sample to the standard curve.
ELISA Type:
Sandwich
Assay Target:
ACADM
Target Species:
Mouse
Research Area:
Fatty acid oxidation; Metabolism; Obesity
Sample Type:
Mouse samples
Detection Range:
0.312-20 ng/mL
Sensitivity:
0.156 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse medium-chain specific acyl-CoA dehydrogenase (ACADM) ELISA kit (0.312-20 ng/mL) is designed for the in vitro quantitative analysis of ACADM levels in mouse samples.
Storage:
Store at 2-8°C.